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anti-RELA antibody :: NF-kappaB p65 Antibody

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Catalog # MBS853735
Unit / Price
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  0.1 mg  /  $215 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

NF-kappaB p65 (RELA), Antibody

Full Product Name

NF-kappaB p65 (Phospho-Ser281) Antibody

Product Synonym Names
NFKB3; nuclear factor NF-kappa-B p65 subunit; p65; RELA; TF65; transcription factor p65
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
164014
3D Structure
ModBase 3D Structure for Q04206
Species Reactivity
Human, Mouse, Rat
Specificity
NF-kappaB p65 (Phospho-Ser281) antibody detects endogenous levels of NF-kappaB p65 only when phosphorylated at serine 281.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human NF-kappaB p65 around the phosphorylation site of serine 281 (E-L-SP-E-P).
All Sites
Human: Ser281
Mouse: Ser281
Rat: Ser281
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-RELA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-RELA antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-RELA antibody
IHC: 1:50-1:100
ELISA: 1:1000

Immunohistochemistry (IHC) of anti-RELA antibody
P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using NF-kappaB p65 (Phospho-Ser281) antibody.
anti-RELA antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for RELA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59,910 Da[Similar Products]
NCBI Official Full Name
transcription factor p65 isoform 2
NCBI Official Synonym Full Names
RELA proto-oncogene, NF-kB subunit
NCBI Official Symbol
NCBI Official Synonym Symbols
p65; NFKB3
  [Similar Products]
NCBI Protein Information
transcription factor p65
UniProt Protein Name
Transcription factor p65
UniProt Synonym Protein Names
Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
NFKB3  [Similar Products]
NCBI Summary for RELA
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Comments for RELA
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681).
Product References and Citations for anti-RELA antibody
Ruben S.M., Science 251:1490-1493(1991). Deloukas P., Hum. Mol. Genet. 2:1895-1900(1993). Lyle R., Gene 138:265-266(1994).

Precautions
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Disclaimer
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