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REN elisa kit :: Prorenin ELISA Kit

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Catalog # MBS135503
Unit / Price
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  1 Kit  /  $540 +1 FREE 8GB USB
  5 Kits  /  $1,980 +2 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Prorenin, ELISA Kit

Popular Item
Full Product Name

Human Prorenin ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Chromosome Location
Chromosome: 1; NC_000001.10 (204123944..204135465, complement). Location: 1q32
OMIM
179820
3D Structure
ModBase 3D Structure for P00797
Host
Multiple
Specificity
This assay recognizes recombinant and natural human prorenin. The factors listed below were prepared at 10 ng/ml in depleted plasma and assayed for cross-reactivity. No significant cross-reactivity was observed. Recombinant human renin Recombinant mouse prorenin
Form/Format
Complete Kit
Samples
Plasma samples
Sensitivity
The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.066-0.074) and calculating the corresponding concentration. The MDD was 0.013 ng/ml.
Intra-assay Precision
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in ten independent assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of REN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for RENpurchase
MBS135503 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Multiple Prorenin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing REN. The ELISA analytical biochemical technique of the MBS135503 kit is based on REN antibody-REN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect REN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, REN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
REN elisa kit
Intended Uses: Human prorenin assay is intended for the quantitative determination of prorenin in plasma samples. Active renin will not be detected by this assay. Prorenin is measured directly by ELISA without pretreatment of samples or conversion to renin [1]. For non-plasma samples such as cell culture media, tissue extracts, or urine samples, use the Human Prorenin ELISA Kit for Non-Plasma Samples (cat # HPRENKT-NP). For research use only.

Principle of the Assay: Human prorenin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-human prorenin primary antibody binds to the captured protein. Only prorenin and not active renin will be detected by the primary antibody. Excess antibody is washed away and bound primary antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of prorenin. The amount of color development is directly proportional to the concentration of prorenin in the sample.

Background: Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. It has been reported that the levels of circulating prorenin (but not renin) are increased in diabetic subjects [2].
Product Categories/Family for REN elisa kit

Typical Testing Data/Standard Curve (for reference only) of REN elisa kit
REN elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS135503. Click to request current manual
NCBI/Uniprot data below describe general gene information for REN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,057 Da
NCBI Official Full Name
renin preproprotein
NCBI Official Synonym Full Names
renin
NCBI Official Symbol
NCBI Official Synonym Symbols
HNFJ2
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NCBI Protein Information
renin; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme
UniProt Protein Name
Renin
UniProt Synonym Protein Names
Angiotensinogenase
Protein Family
UniProt Gene Name
UniProt Entry Name
RENI_HUMAN
NCBI Summary for REN
Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]
UniProt Comments for REN
REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.23.15; Secreted; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; cytoplasm; plasma membrane; extracellular region

Molecular Function: peptidase activity; insulin-like growth factor receptor binding; aspartic-type endopeptidase activity; receptor binding

Biological Process: response to cAMP; hormone-mediated signaling; renin-angiotensin regulation of aldosterone production; drinking behavior; male gonad development; cell maturation; response to lipopolysaccharide; proteolysis; angiotensin maturation; cellular protein metabolic process; beta-amyloid metabolic process; regulation of MAPKKK cascade; regulation of blood pressure; mesonephros development; kidney development

Disease: Renal Tubular Dysgenesis; Hyperuricemic Nephropathy, Familial Juvenile, 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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