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REN elisa kit :: Mouse Renin-1 ELISA Kit

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Catalog # MBS177247
Unit / Price
  96-Strip-Wells  /  $425 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Renin-1, ELISA Kit

Full Product Name

Mouse Renin-1 PicoKine ELISA Kit

Product Synonym Names
Renin-1; renin; Angiotensin forming nzyme; Angiotensinogenase; FLJ10761; HNFJ2; REN; Ren1; RENAA; RENI_HUMAN; Renin; Renin precursor renal; Renin-1; Renin-2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P06281
Species Reactivity
Specificity
Natural and recombinant mouse Renin-1
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of REN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forRENpurchase
MBS177247 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Renin-1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing REN. The ELISA analytical biochemical technique of the MBS177247 kit is based on REN antibody-REN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect REN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, REN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
REN elisa kit
Principle of the assay: mouse Renin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Renin-1 has been precoated onto 96-well plates. Standards(NSO, S72-R402) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Renin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Renin-1 amount of sample captured in plate.
Background: Renin-1 is also known as Ren, Angiotensinogenase or Kidney rennin. It is a member of peptidase A1 family. Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It associated to membranes via binding to ATP6AP2.

Typical Testing Data/Standard Curve (for reference only) of REN elisa kit
Mouse Renin-1 PicoKine ELISA Kit standard curve
REN elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS177247. Click to request current manual
NCBI/Uniprot data below describe general gene information for REN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44,343 Da
NCBI Official Full Name
Renin-1
NCBI Official Synonym Full Names
renin 1 structural
NCBI Official Symbol
NCBI Official Synonym Symbols
Ren; Rnr; Rn-1; Ren-1; Ren-A; Ren1c; Ren1d; D19352
  [Similar Products]
NCBI Protein Information
renin-1; renin b; kidney renin; aspartyl-protease; angiotensinogenase
UniProt Protein Name
Renin-1
UniProt Synonym Protein Names
Angiotensinogenase; Kidney renin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Ren; Ren-1  [Similar Products]
UniProt Entry Name
RENI1_MOUSE
UniProt Comments for REN
REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; EC 3.4.23.15; Protease; Secreted, signal peptide

Cellular Component: extracellular space; membrane; cytoplasm; extracellular region; intracellular

Molecular Function: peptidase activity; insulin-like growth factor receptor binding; hydrolase activity; endopeptidase activity; aspartic-type endopeptidase activity; receptor binding

Biological Process: response to drug; response to cAMP; establishment of blood-nerve barrier; hormone-mediated signaling; cell maturation; renin-angiotensin regulation of aldosterone production; drinking behavior; angiotensin maturation; proteolysis; response to organic substance; regulation of MAPKKK cascade; regulation of blood pressure; renin-angiotensin regulation of blood volume; kidney development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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