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RENIN elisa kit :: Porcine Renin ELISA Kit

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Catalog # MBS013851
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RENIN elisa kit
Product Name

Renin, ELISA Kit

Popular Item
Full Product Name

Porcine Renin ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 1; NC_000001.10 (204123944..204135465, complement). Location: 1q32
OMIM
179820
3D Structure
ModBase 3D Structure for P00797
Species Reactivity
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Samples
Body fluids, tissue homogenates, secretions or feces samples
Assay Type
Quantitative Sandwich
Detection Range
31.2 pg/ml - 1000 pg/ml
Sensitivity
5.0 pg/ml.
Intra-assay Precision
Intra-assay CV (%) is less than 15%
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RENIN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forRENINpurchase
MBS013851 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Renin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RENIN. The ELISA analytical biochemical technique of the MBS013851 kit is based on RENIN antibody-RENIN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RENIN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RENIN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RENIN elisa kit
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of RENIN (hereafter termed "analyte") in undiluted original Porcine body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
Sample Manual Insert of MBS013851. Click to request current manual
NCBI/Uniprot data below describe general gene information for RENIN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,057 Da
NCBI Official Full Name
renin preproprotein
NCBI Official Synonym Full Names
renin
NCBI Official Symbol
NCBI Official Synonym Symbols
HNFJ2
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NCBI Protein Information
renin; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme
UniProt Protein Name
Renin
UniProt Synonym Protein Names
Angiotensinogenase
Protein Family
UniProt Gene Name
UniProt Entry Name
RENI_HUMAN
NCBI Summary for RENIN
Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]
UniProt Comments for RENIN
REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Protease; EC 3.4.23.15

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; cytoplasm; plasma membrane; extracellular region

Molecular Function: peptidase activity; insulin-like growth factor receptor binding; aspartic-type endopeptidase activity; receptor binding

Biological Process: response to cAMP; hormone-mediated signaling; cell maturation; drinking behavior; male gonad development; renin-angiotensin regulation of aldosterone production; response to lipopolysaccharide; proteolysis; angiotensin maturation; cellular protein metabolic process; regulation of MAPKKK cascade; regulation of blood pressure; beta-amyloid metabolic process; mesonephros development; kidney development

Disease: Renal Tubular Dysgenesis; Hyperuricemic Nephropathy, Familial Juvenile, 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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