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Hin6I Restriction Enzyme

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Catalog # MBS638354
Unit / Price
  2000 Units  /  $350 +1 FREE 8GB USB
Hin6I restriction enzyme
Product Name

Hin6I, Restriction Enzyme

Popular Item
Also Known As

Hin6I (HhaI*)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Purity/Purification
Molecular Biology Grade
Dilution Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods the Storage Buffer should be used.
Digestion of Agarose-embedded DNA
A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Compatible Ends
AciI, Bsp119I, Bsu15I, Hin1I, HpaII, MaeII, MspI, NarI, Psp1406I, TaqI, XmiI
Methylation Effects
Hin6I does not cut Gm5CGC. Blocked by CG methylation.
pACYC177
16
pACYC184
26
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Lambda
215
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with Hin6I, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 2.0uM. More than 95% of these can be recut.
M13mp18/19
26
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Hin6I.
pBR322
31
PhiX174
18
pTZ19R/U
20
pUC18/19
17
pUC57
17
Stability During Prolonged Incubation
A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer
10mM potassium phosphate (pH 7.4 at 25 degree C), 100mM NaCl, 1mM EDTA, 7mM 2-mercaptoethanol, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation
Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.
Preparation and Storage
-20 degree C
Other Notes
Small volumes of Hin6I restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Hin6I restriction enzyme
5'-G^C G C-3'
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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