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HinfI Restriction Enzyme

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Catalog # MBS638389
Unit / Price
  2000 Units  /  $315 +1 FREE 8GB USB
HinfI restriction enzyme
Product Name

HinfI, Restriction Enzyme

Popular Item
Also Known As

HinfI

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Purity/Purification
Molecular Biology Grade
Methylation Effects
Dam: never overlaps-no effect
Dcm: never overlaps-no effect
CpG: may overlap-cleavage impaired
EcoKI: never overlaps-no effect
EcoBI: may overlap-blocked
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded or double-stranded labeled oligonucleotide occured during incubation with 10 units of HinfI for 4 hours.
Lambda
148
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with HinfI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 1.3uM. More than 95% of these can be recut.
M13mp18/19
27
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion with HinfI (10u/ug lambda DNA x 16 hours).
pBR322
10
PhiX174
21
pTZ19R/U
9
pUC18/19
6
pUC57
5
Stability During Prolonged Incubation
A minimum of 0.1 units of HinfI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Preparation and Storage
-20 degree C
Other Notes
Small volumes of HinfI restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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