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RGMA elisa kit :: Human Repulsive guidance molecule A (RGMA) ELISA Kit

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Catalog # MBS7208752
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
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 Typical Testing Data/Standard Curve (for reference only)
Product Name

Repulsive guidance molecule A (RGMA), ELISA Kit

Popular Item
Full Product Name

Human Repulsive guidance molecule A (RGMA) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
607362
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of RGMA. No significant cross-reactivity or interference between RGMA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RGMA and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RGMA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for RGMApurchase
MBS7208752 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Repulsive guidance molecule A (RGMA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RGMA. The ELISA analytical biochemical technique of the MBS7208752 kit is based on RGMA antibody-RGMA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RGMA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RGMA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RGMA elisa kit
Intended Uses: This RGMA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RGMA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: RGMA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RGMA antibody and an RGMA-HRP conjugate. The assay sample and buffer are incubated together with RGMA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RGMA concentration since RGMA from samples and RGMA-HRP conjugate compete for the anti-RGMA antibody binding site. Since the number of sites is limited, as more sites are occupied by RGMA from the sample, fewer sites are left to bind RGMA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RGMA concentration in each sample is interpolated from this standard curve.
Product Categories/Family for RGMA elisa kit

Typical Testing Data/Standard Curve (for reference only) of RGMA elisa kit
RGMA elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7208752. Click to request current manual
NCBI/Uniprot data below describe general gene information for RGMA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
50,035 Da
NCBI Official Full Name
repulsive guidance molecule A isoform 2
NCBI Official Synonym Full Names
repulsive guidance molecule family member a
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
repulsive guidance molecule A; RGM domain family, member A
UniProt Protein Name
Repulsive guidance molecule A
UniProt Synonym Protein Names
RGM domain family member A
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
RGMA_HUMAN
NCBI Summary for RGMA
This gene encodes a member of the repulsive guidance molecule family. The encoded protein is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. This protein may also function as a tumor suppressor in some cancers. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
UniProt Comments for RGMA
RGMA: Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8. Belongs to the repulsive guidance molecule (RGM) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: endoplasmic reticulum; plasma membrane

Molecular Function: coreceptor activity

Biological Process: BMP signaling pathway; axon guidance
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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