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RHO elisa kit :: Human rhodopsin ELISA Kit

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Catalog # MBS910995
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

rhodopsin, ELISA Kit

Popular Item
Full Product Name

Human Rhodopsin, RHO ELISA Kit

Product Synonym Names
Human Rhodopsin (RHO) ELISA kit; CSNBAD1; MGC138309; MGC138311; OPN2; RP4; opsin 2; rod pigment; retinitis pigmentosa 4; autosomal dominant; rhodopsin (opsin 2; rod pigment) (retinitis pigmentosa 4; autosomal dominant) ; rhodopsin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS910995 Testing Data
OMIM
613731
3D Structure
ModBase 3D Structure for P08100
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human RHO. No significant cross-reactivity or interference between human RHO and analogues was observed.
Samples
serum, plasma, tissue homogenates and cell lysates
Assay Type
Quantitative Sandwich
Detection Range
12.5 pg/ml -800 pg/ml
Sensitivity
<3.12 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RHO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forRHOpurchase
MBS910995 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the rhodopsin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RHO. The ELISA analytical biochemical technique of the MBS910995 kit is based on RHO antibody-RHO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RHO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RHO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RHO elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RHO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RHO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RHO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RHO bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of RHO elisa kit
RHO elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS910995. Click to request current manual
NCBI/Uniprot data below describe general gene information for RHO. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
38,893 Da
NCBI Official Full Name
rhodopsin
NCBI Official Synonym Full Names
rhodopsin
NCBI Official Symbol
NCBI Official Synonym Symbols
RP4; OPN2; CSNBAD1
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NCBI Protein Information
rhodopsin; opsin-2; opsin 2, rod pigment
UniProt Protein Name
Rhodopsin
UniProt Synonym Protein Names
Opsin-2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
OPSD_HUMAN
NCBI Summary for RHO
Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq, Jul 2008]
UniProt Comments for RHO
Rhodopsin: a G-protein coupled receptor. The light-absorbing visual pigment in Rod photoreceptor cells. Mediates vision in dim light. Consists of the apoprotein, opsin, covalently linked to cis-retinal. Defects in RHO are a cause of autosomal retinitis pigmentosa and congenital stationary night blindness.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q21-q24

Cellular Component: Golgi membrane; Golgi apparatus; photoreceptor outer segment; photoreceptor inner segment; integral to plasma membrane; plasma membrane; rough endoplasmic reticulum membrane; intercellular junction

Molecular Function: G-protein coupled receptor activity; protein binding; spectrin binding; metal ion binding; photoreceptor activity; retinal binding

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; G-protein coupled receptor protein signaling pathway; red, far-red light phototransduction; regulation of rhodopsin mediated signaling; visual perception; retina development in camera-type eye; organelle organization and biogenesis; retinoid metabolic process; protein amino acid phosphorylation; protein-chromophore linkage

Disease: Fundus Albipunctatus; Night Blindness, Congenital Stationary, Autosomal Dominant 1; Retinitis Pigmentosa 4
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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