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anti-Rho Kinase/ROCKII (Paired T396) antibody :: Rabbit Rho Kinase/ROCKII (Paired T396) Antibody

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Catalog # MBS462122
Unit / Price
  0.1 mg  /  $285 +1 FREE 8GB USB
Testing Data
Product Name

Rho Kinase/ROCKII (Paired T396), Antibody

Also Known As

Rabbit anti ROCKII (Paired Thr396), Non-Phospho

Product Synonym Names
Rabbit anti-ROCKII (Paired T396) Non-phosphospecific; Rho Kinase; Rho-associated kinase-alpha (ROKalpha); Rho-associated; coiled-coil containing protein kinase II (ROCKII)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Chicken, Bovine, Canine
Specificity
This antibody recognizes ~165 kDa of human ROCKII without a phosphorylation site ofThr396. It does not react to phosphorylated protein. This antibody also reacts with mouse and rat. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (lot specific)
Antigen Preparation
A synthetic peptide corresponding to the epitope -VETFP- without a phosphorylation site Thr396 of human RockII protein. This sequence is identical among human, mouse, rat, chicken, dog and bovine.
Positive Control
3T3 cell
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Other Notes
Small volumes of anti-Rho Kinase/ROCKII (Paired T396) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Rho Kinase/ROCKII (Paired T396) antibody
Rho A-dependent kinase (ROCKII), or ROK-alpha, is identified a serine/threonine kinase, a downstream effector of the small GTPase Rho, mediating effects of Rho on the actin cytoskeleton, formation of actin stress fibers and focal adhesions, ROCKII alters cardiac myofilament response to Ca2+ by mechanisms involving phosphorylation of thin filament proteins. ROCK-II induces a depression in maximum ATPase rate and tension, which is associated with phosphorylation of troponin T (TnT), troponin I (TnI), and myosin-binding protein C (C-protein). The phosphorylation of Rho-kinase at Thr 249, Thr 396 plays important role in G-protein signaling..
Applications Tested/Suitable for anti-Rho Kinase/ROCKII (Paired T396) antibody
ELISA, Western Blot, Immunohistochemistry
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 0.5-2 ug/ml

Molecular Weight
165 kDa
Product References and Citations for anti-Rho Kinase/ROCKII (Paired T396) antibody
Susan Vahebi, et al, Functional Effects of Rho-Kinase-Dependent Phosphorylation of Specific Sites on Cardiac Troponin. Circulation Research. 2005;96:740-747.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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