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anti-Rho Kinase/ROCKII (pT396) antibody :: Rabbit Rho Kinase/ROCKII (pT396) Antibody

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Catalog # MBS462125
Unit / Price
  0.1 mg  /  $285 +1 FREE 8GB USB
Testing Data
Product Name

Rho Kinase/ROCKII (pT396), Antibody

Popular Item
Full Product Name

Rabbit anti Phospho-ROCKII (pThr 396) antibody

Product Synonym Names
Rabbit anti-ROCKII (pThr396); Rho Kinase; Rho-associated kinase-alpha (ROKalpha); Rho-associated; coiled-coil containing protein kinase II (ROCKII)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human, Mouse, Rat, Chicken, Bovine, Canine
This antibody recognizes ~165 kDa of human ROCKII at a phosphorylation site ofThr396. It does not react to non-phosphorylated protein. This antibody also reacts with mouse and rat. The other species are not tested.
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Size: 100 ug/200 ul (lot specific)
Antigen Preparation
A synthetic peptide corresponding to the epitope -VETFP- with a single phosphorylation site Thr396 of human RockII protein. This sequence is identical among human, mouse, rat, chicken, dog and bovine.
Positive Control
Conditioned 3T3 cell
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 20 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Other Notes
Small volumes of anti-Rho Kinase/ROCKII (pT396) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Rho Kinase/ROCKII (pT396) antibody
Rho A-dependent kinase (ROCKII), or ROK-alpha, is identified a serine/threonine kinase, a downstream effector of the small GTPase Rho, mediating effects of Rho on the actin cytoskeleton, formation of actin stress fibers and focal adhesions, ROCKII alters cardiac myofilament response to Ca2+ by mechanisms involving phosphorylation of thin filament proteins. ROCK-II induces a depression in maximum ATPase rate and tension, which is associated with phosphorylation of troponin T (TnT), troponin I (TnI), and myosin-binding protein C (C-protein). The phosphorylation of Rho-kinase at Thr 249, Thr 396 plays important role in G-protein signaling..
Applications Tested/Suitable for anti-Rho Kinase/ROCKII (pT396) antibody
ELISA, Western Blot, Immunohistochemistry
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 0.5-2 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.

Molecular Weight
165 kDa
Product References and Citations for anti-Rho Kinase/ROCKII (pT396) antibody
Susan Vahebi, et al, Functional Effects of Rho-Kinase-Dependent Phosphorylation of Specific Sites on Cardiac Troponin. Circulation Research. 2005;96:740-747.

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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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