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RIPK3 elisa kit :: Human Receptor Interacting Serine Threonine Kinase 3 (RIPK3) ELISA Kit

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Catalog # MBS456917
Unit / Price
  48-Strip-Wells  /  $400 +1 FREE 8GB USB
  96-Strip-Wells  /  $535 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,425 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,710 +5 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

Receptor Interacting Serine Threonine Kinase 3 (RIPK3), ELISA Kit

Popular Item
Also Known As

Human Receptor Interacting Serine Threonine Kinase 3 (RIPK3) ELISA Kit

Product Synonym Names
RIP3; RIP-like protein kinase 3; Receptor-interacting protein 3
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
518
OMIM
AF156884 mRNA
3D Structure
ModBase 3D Structure for Q9Y572
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of RIPK3. No significant cross-reactivity or interference between RIPK3 and analogues was observed.
Samples
Tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
31.2-2000pg/mL
Sensitivity
<11.7pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RIPK3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RIPK3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RIPK3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forRIPK3purchase
MBS456917 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Receptor Interacting Serine Threonine Kinase 3 (RIPK3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RIPK3. The ELISA analytical biochemical technique of the MBS456917 kit is based on RIPK3 antibody-RIPK3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RIPK3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RIPK3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RIPK3 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of RIPK3 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RIPK3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RIPK3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RIPK3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RIPK3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of RIPK3 elisa kit
RIPK3 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS456917. Click to request current manual
NCBI/Uniprot data below describe general gene information for RIPK3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,326 Da
NCBI Official Full Name
receptor-interacting serine/threonine-protein kinase 3
NCBI Official Synonym Full Names
receptor interacting serine/threonine kinase 3
NCBI Official Symbol
RIPK3  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
receptor-interacting serine/threonine-protein kinase 3
UniProt Protein Name
Receptor-interacting serine/threonine-protein kinase 3
UniProt Synonym Protein Names
RIP-like protein kinase 3; Receptor-interacting protein 3; RIP-3
UniProt Gene Name
RIPK3  [Similar Products]
UniProt Synonym Gene Names
RIP3; RIP-3  [Similar Products]
NCBI Summary for RIPK3
The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008]
UniProt Comments for RIPK3
RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; RIPK family; TKL group

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: cytosol; mitochondrion; plasma membrane

Molecular Function: ATP binding; identical protein binding; NF-kappaB-inducing kinase activity; protein binding; protein complex binding; protein kinase activity; protein serine/threonine kinase activity; transcription coactivator activity

Biological Process: activation of protein kinase activity; amyloid fibril formation; cellular protein modification process; I-kappaB kinase/NF-kappaB signaling; lymph node development; necroptotic process; NIK/NF-kappaB signaling; positive regulation of intrinsic apoptotic signaling pathway; positive regulation of ligase activity; positive regulation of NF-kappaB transcription factor activity; positive regulation of oxidoreductase activity; positive regulation of phosphatase activity; positive regulation of protein deacetylation; positive regulation of reactive oxygen species metabolic process; protein autophosphorylation; protein heterooligomerization; protein homooligomerization; regulation of activated T cell proliferation; regulation of adaptive immune response; regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation; regulation of interferon-gamma production; regulation of T cell mediated cytotoxicity; signal transduction; spleen development; T cell differentiation in thymus; T cell homeostasis; thymus development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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