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RNASE3 elisa kit :: Human Ribonuclease A3 (RNASE3) ELISA Kit

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Catalog # MBS455305
Unit / Price
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  48-Strip-Wells  /  $385 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Ribonuclease A3 (RNASE3), ELISA Kit

Popular Item
Full Product Name

Human Ribonuclease A3 (RNASE3) ELISA Kit

Product Synonym Names
Rnase-A3; RnaseA3; ECP; RNS3; Ribonuclease,RNase A Family 3; Eosinophil Cationic Protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
X15161 mRNA
3D Structure
ModBase 3D Structure for P12724
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of RNASE3. No significant cross-reactivity or interference between RNASE3 and analogues was observed.
Samples
Serum, plasma, cell lysates and other biological fluids.
Assay Type
Sandwich
Detection Range
78.12-5000pg/mL
Sensitivity
28.43pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RNASE3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RNASE3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RNASE3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for RNASE3purchase
MBS455305 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ribonuclease A3 (RNASE3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RNASE3. The ELISA analytical biochemical technique of the MBS455305 kit is based on RNASE3 antibody-RNASE3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RNASE3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RNASE3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RNASE3 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RNASE3 in human serum, plasma, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RNASE3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RNASE3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNASE3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RNASE3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of RNASE3 elisa kit
RNASE3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS455305. Click to request current manual
NCBI/Uniprot data below describe general gene information for RNASE3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18,385 Da
NCBI Official Full Name
eosinophil cationic protein
NCBI Official Synonym Full Names
ribonuclease A family member 3
NCBI Official Symbol
RNASE3  [Similar Products]
NCBI Official Synonym Symbols
ECP; RAF1; RNS3
  [Similar Products]
NCBI Protein Information
eosinophil cationic protein
UniProt Protein Name
Eosinophil cationic protein
UniProt Synonym Protein Names
Ribonuclease 3; RNase 3
UniProt Gene Name
RNASE3  [Similar Products]
UniProt Synonym Gene Names
ECP; RNS3; ECP; RNase 3  [Similar Products]
NCBI Summary for RNASE3
The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]
UniProt Comments for RNASE3
Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with RNASE3 elisa kit
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