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RNASET2 elisa kit :: Human Ribonuclease T2 (RNASET2) ELISA Kit

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Catalog # MBS2020833 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Ribonuclease T2 (RNASET2), ELISA Kit

Popular Item
Full Product Name

Ribonuclease T2 (RNASET2) ELISA Kit

Product Synonym Names
Rnase-T2; RNASE6PL; RNASE6-PL; Ribonuclease 6
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q9CQ01
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Ribonuclease T2 (RNASET2).
No significant cross-reactivity or interference between Ribonuclease T2 (RNASET2) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.061ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Ribonuclease T2 (RNASET2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Ribonuclease T2 (RNASET2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RNASET2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for RNASET2purchase
MBS2020833 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ribonuclease T2 (RNASET2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RNASET2. The ELISA analytical biochemical technique of the MBS2020833 kit is based on RNASET2 antibody-RNASET2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RNASET2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RNASET2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RNASET2 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease T2 (RNASET2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease T2 (RNASET2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease T2 (RNASET2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribonuclease T2 (RNASET2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for RNASET2 elisa kit

Typical Testing Data/Standard Curve (for reference only) of RNASET2 elisa kit
RNASET2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2020833. Click to request current manual
NCBI/Uniprot data below describe general gene information for RNASET2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
29,609 Da
NCBI Official Full Name
ribonuclease T2
NCBI Official Synonym Full Names
ribonuclease T2A
NCBI Official Symbol
Rnaset2a  [Similar Products]
NCBI Official Synonym Symbols
C79750; Rnaset2; RNASE6PL; 0610007O07Rik; 4833423A10Rik; 4930532K22Rik
  [Similar Products]
NCBI Protein Information
ribonuclease T2
UniProt Protein Name
Ribonuclease T2
UniProt Synonym Protein Names
Ribonuclease 6
Protein Family
UniProt Gene Name
Rnaset2  [Similar Products]
UniProt Synonym Gene Names
Rnase6pl  [Similar Products]
UniProt Entry Name
RNT2_MOUSE
UniProt Comments for RNASET2
RNASET2: Has ribonuclease activity, with higher activity at acidic pH. May play a role in cellular RNA catabolism. Defects in RNASET2 are the cause of leukoencephalopathy cystic without megalencephaly (LCWM). An infantile- onset syndrome of cerebral leukoencephalopathy. Affected newborns develop microcephaly and neurologic abnormalities including psychomotor impairment, seizures and sensorineural hearing impairment. The brain shows multifocal white matter lesions, anterior temporal lobe subcortical cysts, pericystic abnormal myelination, ventriculomegaly and intracranial calcifications. Belongs to the RNase T2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Ribonuclease; EC 3.1.27.-; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; endoplasmic reticulum lumen; lysosome

Molecular Function: ribonuclease activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with RNASET2 elisa kit
 Products by Pathway  Pathway Diagram
 MRNA Processing Pathway antibodies  MRNA Processing Pathway Diagram
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