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RNASET2 elisa kit :: Rat RNASET2 ELISA Kit

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Catalog # MBS2509900
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

RNASET2, ELISA Kit

Popular Item
Full Product Name

Rat RNASET2 (Ribonuclease T2) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
612944
3D Structure
ModBase 3D Structure for O00584
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat RNASET2. No significant cross-reactivity or interference between Rat RNASET2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat RNASET2 and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RNASET2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forRNASET2purchase
MBS2509900 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the RNASET2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RNASET2. The ELISA analytical biochemical technique of the MBS2509900 kit is based on RNASET2 antibody-RNASET2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RNASET2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RNASET2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RNASET2 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RNASET2 in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat RNASET2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat RNASET2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RNASET2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat RNASET2. You can calculate the concentration of Rat RNASET2 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of RNASET2 elisa kit
RNASET2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2509900. Click to request current manual
NCBI/Uniprot data below describe general gene information for RNASET2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
14,000 Da
NCBI Official Full Name
ribonuclease T2
NCBI Official Synonym Full Names
ribonuclease T2
NCBI Official Symbol
RNASET2  [Similar Products]
NCBI Official Synonym Symbols
RNASE6PL; bA514O12.3
  [Similar Products]
NCBI Protein Information
ribonuclease T2; ribonuclease 6
UniProt Protein Name
Ribonuclease T2
UniProt Synonym Protein Names
Ribonuclease 6
Protein Family
UniProt Gene Name
RNASET2  [Similar Products]
UniProt Synonym Gene Names
RNASE6PL  [Similar Products]
UniProt Entry Name
RNT2_HUMAN
NCBI Summary for RNASET2
This ribonuclease gene is a novel member of the Rh/T2/S-glycoprotein class of extracellular ribonucleases. It is a single copy gene that maps to 6q27, a region associated with human malignancies and chromosomal rearrangement. [provided by RefSeq, Jul 2008]
UniProt Comments for RNASET2
RNASET2: Has ribonuclease activity, with higher activity at acidic pH. May play a role in cellular RNA catabolism. Defects in RNASET2 are the cause of leukoencephalopathy cystic without megalencephaly (LCWM). An infantile- onset syndrome of cerebral leukoencephalopathy. Affected newborns develop microcephaly and neurologic abnormalities including psychomotor impairment, seizures and sensorineural hearing impairment. The brain shows multifocal white matter lesions, anterior temporal lobe subcortical cysts, pericystic abnormal myelination, ventriculomegaly and intracranial calcifications. Belongs to the RNase T2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 3.1.27.-; Ribonuclease; Secreted; RNA-binding

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: lysosomal lumen; extracellular space; endoplasmic reticulum lumen; lysosome; extracellular region

Molecular Function: ribonuclease T2 activity; RNA binding; ribonuclease activity

Biological Process: RNA catabolic process

Disease: Leukoencephalopathy, Cystic, Without Megalencephaly
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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