• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ROS1 elisa kit :: Rat C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2021712 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  24-Strip-Wells  /  $285 +1 FREE 8GB USB
  48-Strip-Wells  /  $530 +1 FREE 8GB USB
  96-Strip-Wells  /  $715 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,910 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,280 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1), ELISA Kit

Full Product Name

C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) ELISA Kit

Product Synonym Names
MCF3; ROS; c-Ros-1; v-Ros Avian UR2 Sarcoma Virus Oncogene Homolog 1; Proto-oncogene tyrosine-protein kinase ROS; Receptor tyrosine kinase c-ros oncogene 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
165020
3D Structure
ModBase 3D Structure for P08922
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1).
No significant cross-reactivity or interference between C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
15.62-1000pg/mL
Sensitivity
< 6.6pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ROS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forROS1purchase
MBS2021712 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ROS1. The ELISA analytical biochemical technique of the MBS2021712 kit is based on ROS1 antibody-ROS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ROS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ROS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ROS1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for ROS1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of ROS1 elisa kit
ROS1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2021712. Click to request current manual
NCBI/Uniprot data below describe general gene information for ROS1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
263,915 Da
NCBI Official Full Name
C-ros oncogene 1, receptor tyrosine kinase
NCBI Official Synonym Full Names
ROS proto-oncogene 1 , receptor tyrosine kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
ROS; MCF3; c-ros-1
  [Similar Products]
NCBI Protein Information
proto-oncogene tyrosine-protein kinase ROS
UniProt Protein Name
Proto-oncogene tyrosine-protein kinase ROS
UniProt Synonym Protein Names
Proto-oncogene c-Ros; Proto-oncogene c-Ros-1; Receptor tyrosine kinase c-ros oncogene 1; c-Ros receptor tyrosine kinase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
MCF3; ROS  [Similar Products]
UniProt Entry Name
ROS1_HUMAN
NCBI Summary for ROS1
This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008]
UniProt Comments for ROS1
ROS: a proto-oncogenic receptor tyrosine kinase whose expression is tightly restricted during development. Normally expressed in adult murine and human epithelial cells of the epididymis. Transgenic mice lacking the c-ros gene are infertile. Ectopic expression of c-Ros has been reported in meningiomas and astrocytomas. Is up-regulated in human glioma: 30% of malignant glioma tumors are ROS positive. An oncogenic fusion protein between PIST (aka FIG) and ROS, resulting from an intra-chromosomal homozygous deletion of 240 kilobases on 6q21, is found in glioblastoma multiform. PIST (aka FIG) is a peripheral membrane protein associated with the Golgi apparatus. Unlike other fusion RTK oncogenes, the mechanism of activation of PIST-ROS does not appear to be dimerization: the PIST-ROS fusion protein appears to be monomeric in vivo. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region of PIST, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. c-ROS may also be activated epigenetically, suggesting caution when using 5-aza-dC for treating glioma.

Protein type: Oncoprotein; Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; EC 2.7.10.1; Protein kinase, TK; TK group; Sev family

Chromosomal Location of Human Ortholog: 6q22

Cellular Component: membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; protein phosphatase binding; ATP binding

Biological Process: cell proliferation; peptidyl-tyrosine phosphorylation; spermatogenesis; cell growth; columnar/cuboidal epithelial cell development; cell differentiation; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of TOR signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000