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RPL13 elisa kit :: Human 60S ribosomal protein L13 ELISA Kit

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Catalog # MBS765773
Unit / Price
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  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Product Name

60S ribosomal protein L13 (RPL13), ELISA Kit

Full Product Name

Human 60S ribosomal protein L13 ELISA Kit

Product Synonym Names
RPL13/Breast basic conserved protein 1/RPL13/BBC1/OK/SW-cl.46
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
113703
Species Reactivity
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.625-40ng/ml
Sensitivity
0.375ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RPL13 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for RPL13purchase
MBS765773 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 60S ribosomal protein L13 (RPL13) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RPL13. The ELISA analytical biochemical technique of the MBS765773 kit is based on RPL13 antibody-RPL13 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RPL13 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RPL13. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RPL13 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human RPL13 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RPL13 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RPL13 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman RPL13 can be calculated.

Typical Testing Data of RPL13 elisa kit
RPL13 elisa kit Typical Testing Data image
Typical Standard Curve of RPL13 elisa kit
RPL13 elisa kit Typical Standard Curve image
Sample Manual Insert of MBS765773. Click to request current manual
NCBI/Uniprot data below describe general gene information for RPL13. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18,845 Da
NCBI Official Full Name
60S ribosomal protein L13 isoform 3
NCBI Official Synonym Full Names
ribosomal protein L13
NCBI Official Symbol
RPL13  [Similar Products]
NCBI Official Synonym Symbols
L13; BBC1; D16S44E; D16S444E
  [Similar Products]
NCBI Protein Information
60S ribosomal protein L13
UniProt Protein Name
60S ribosomal protein L13
UniProt Synonym Protein Names
Breast basic conserved protein 1
Protein Family
UniProt Gene Name
RPL13  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
RL13_HUMAN
NCBI Summary for RPL13
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13E family of ribosomal proteins. It is located in the cytoplasm. This gene is expressed at significantly higher levels in benign breast lesions than in breast carcinomas. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2011]
UniProt Comments for RPL13
RPL13: a ribosomal protein. A component of the 60S ribosomal subunit. Reduced expression observed in breast cancer.

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 16q24.3|17p11.2

Cellular Component: cytosol; membrane; nucleus

Molecular Function: protein binding; RNA binding; structural constituent of ribosome

Biological Process: cellular protein metabolic process; gene expression; mRNA catabolic process, nonsense-mediated decay; selenium metabolic process; selenocysteine metabolic process; SRP-dependent cotranslational protein targeting to membrane; translation; translational elongation; translational initiation; translational termination; viral infectious cycle; viral reproduction; viral transcription
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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