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RPL35A elisa kit :: Rat 60S ribosomal protein L35a ELISA Kit

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Catalog # MBS281166
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RPL35A elisa kit
Product Name

60S ribosomal protein L35a (RPL35A), ELISA Kit

Full Product Name

Rat 60S ribosomal protein L35a (RPL35A) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P04646
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat RPL35A. No significant cross-reactivity or interference between Rat RPL35A and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RPL35A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for RPL35Apurchase
MBS281166 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 60S ribosomal protein L35a (RPL35A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RPL35A. The ELISA analytical biochemical technique of the MBS281166 kit is based on RPL35A antibody-RPL35A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RPL35A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RPL35A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RPL35A elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate RPL35A in samples. An antibody specific for RPL35A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRPL35A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RPL35A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RPL35A bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for RPL35A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
12,554 Da
NCBI Official Full Name
60S ribosomal protein L35a
NCBI Official Synonym Full Names
ribosomal protein L35a
NCBI Official Symbol
Rpl35a  [Similar Products]
NCBI Protein Information
60S ribosomal protein L35a
UniProt Protein Name
60S ribosomal protein L35a
Protein Family
UniProt Gene Name
Rpl35a  [Similar Products]
NCBI Summary for RPL35A
ribosomal protein that binds tRNA [RGD, Feb 2006]
UniProt Comments for RPL35A
RPL35A: Required for the proliferation and viability of hematopoietic cells. Plays a role in 60S ribosomal subunit formation. The protein was found to bind to both initiator and elongator tRNAs and consequently was assigned to the P site or P and A site. Defects in RPL35A are the cause of Diamond-Blackfan anemia type 5 (DBA5). DBA5 is a form of Diamond- Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein L35Ae family.

Protein type: RNA-binding; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 12p11

Molecular Function: structural constituent of ribosome; tRNA binding

Biological Process: ribosomal large subunit biogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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