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RPL8 elisa kit :: Human 60S ribosomal protein L8 (RPL8) ELISA Kit

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Catalog # MBS7219850
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Typical Testing Data/Standard Curve
Product Name

60S ribosomal protein L8 (RPL8), ELISA Kit

Popular Item
Full Product Name

Human 60S ribosomal protein L8 (RPL8) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
604177
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of RPL8. No significant cross-reactivity or interference between RPL8 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPL8 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RPL8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for RPL8purchase
MBS7219850 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 60S ribosomal protein L8 (RPL8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RPL8. The ELISA analytical biochemical technique of the MBS7219850 kit is based on RPL8 antibody-RPL8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RPL8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RPL8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RPL8 elisa kit
Intended Uses: This RPL8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RPL8. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||RPL8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPL8 antibody and an RPL8-HRP conjugate. The assay sample and buffer are incubated together with RPL8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPL8 concentration since RPL8 from samples and RPL8-HRP conjugate compete for the anti-RPL8 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPL8 from the sample, fewer sites are left to bind RPL8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPL8 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for RPL8 elisa kit

Typical Testing Data/Standard Curve of RPL8 elisa kit
RPL8 elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS7219850. Click to request current manual
NCBI/Uniprot data below describe general gene information for RPL8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
28,025 Da
NCBI Official Full Name
60S ribosomal protein L8
NCBI Official Synonym Full Names
ribosomal protein L8
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
60S ribosomal protein L8
UniProt Protein Name
60S ribosomal protein L8
Protein Family
UniProt Gene Name
UniProt Entry Name
RL8_HUMAN
NCBI Summary for RPL8
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Comments for RPL8
RPL8: a ribosomal protein of the L2P family. Located in the cytoplasm. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit. Associates with 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface.

Protein type: Ribosomal; Translation; RNA-binding

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: focal adhesion; membrane; cytoplasm; nucleolus; cytosol

Molecular Function: rRNA binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; translational termination; viral infectious cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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