MyBioSource manufactures and supplies human and mammalian proteins as well as proteins from a myriad of lower organisms. Proteins may purified from natural sources or created artificially. Proteins created artificially are typically referred to as recombinant proteins or proteins manufactured through genetic engineering. Genetic engineering involves cloning genes into plasmids to generate recombinant DNA, which is sometimes referred to as "chimera" DNA when the recombinant DNA originates from more that species.
Jelly fish GFP, a protein composed of 238 amino acid residues, can cloned into a plasmid along with genes for human or mammalian proteins to generate a chimera recombinant protein with a GFP tag. This GFP technology is used for detectability of a protein that exhibits bright green GFP fluorescence when exposed to light. The His peptide tag, containing ~6 amino acids, and the GST peptide tag, containing ~211 amino acids are also among the most common protein tags used in genetic engineering for proteins created artificially.
Common hosts for proteins
E. coli, yeast, baculovirus, and mammalian hosts among the most common hosts for proteins created artificially. We should also familiarize ourselves with the term 'metabolic engineering' referring to the use of genetic engineering to optimize the metabolic machinery of the hosts to render them efficient manufacturers of high quality protein. Commercially manufactured proteins are expressed from hosts optimized through metabolic engineering. The expressed proteins are then purified by highly developed proprietary methods resulting in high quality human and mammalian proteins or for that matter proteins from any species of choice.
Even though we speak of proteins created artificially, we should keep in mind that proteins are simply a group of organic molecules and in this regard may function and act similarly to the natural protein. The specific functionality of a recombinant protein often must be empirically determined. There are many factors which influence the functionality of a protein.
Recombinant protein expression and purification
This includes the host employed for recombinant protein expression as well as the type of protein being expressed. Different hosts have different cellular machinery and capabilities for protein modifications. Likewise, different proteins such as protein hormones, peptide hormones, glycosylated proteins, phosphorylated proteins, and enzyme proteins all have different needs for functionality. Likewise, many applications employed for recombinant human and mammalian proteins, as well as proteins from other species, may not rely on biological functionality.
MyBioSource offers free consultation services to help you determine your recombinant protein needs, including custom design. Protein manufacture services also include, upon request, tag removal, endotoxin removal and aseptic manufacture.
Here is an overview of the recombinant protein manufacture, purification and quality control processes:
1. Cloning Stage: We perform gene sequencing to ensure completely correct amino acid sequence.
2. Protein PurificationStage: Our protein is purified by our unique chromatographic technique under mild conditions to avoid activity loss, and our protein is soluble.
3. Protein Detection Stage: We perform SDS-PAGE identification to ensure 90% or higher purity. Upon request, we also perform western blot analysis of the tag antibody.
4. We also an antibody service as an add-on to your recombinant protein, where you protein is used as immunogen to generate a rabbit polyclonal antibody.
Custom Protein Productions
We offer the flexibility of expression in E. Coli, Yeast, Baculovirus or Mammalian Cell hosts. Proteins are produced under mild conditions to avoid activity loss. Additional services are also available on upon request.
A disintegrin and metalloproteinase with thrombospondin motifs 13
ADAMTS13; Gm710; vWF-CP; ADAM-TS13; ADAMTS-13
von Willebrand factor-cleaving protease; A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 1 preproprotein; a disintegrin-like and metallopeptidase (reprolysin type) with throm...
Host: E Coli
>95% by SDS-PAGE
ELISA (EIA), Western Blot (WB), Affinity Purification (AP), Protein Array
A disintegrin and metalloproteinase with thrombospondin motifs 5; ADAM-TS 5; ADAMTS-5; EC=3.4.24.-; ADMP-2; Aggrecanase-2; Implantin; a disintegrin-like and metallopeptidase (reprolysin type) with thr...
Host: E Coli or Yeast or Baculovirus or Mammalian Cell
AKAP7 Human; A Kinase Anchor Protein 7 Human Recombinant; A kinase (PRKA) anchor protein 7; A-kinase anchor protein 18 kDa; A-kinase anchor protein 9; kDa PRKA7 isoforms alpha/beta; PRKA7 isoform gamm...