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RPS28 elisa kit :: Human 40S ribosomal protein S28 (RPS28) ELISA Kit

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Catalog # MBS7225690
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

40S ribosomal protein S28 (RPS28), ELISA Kit

Popular Item
Also Known As

Human 40S ribosomal protein S28 (RPS28) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
603685
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of RPS28. No significant cross-reactivity or interference between RPS28 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPS28 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This RPS28 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RPS28. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RPS28 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forRPS28purchase
MBS7225690 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 40S ribosomal protein S28 (RPS28) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RPS28. The ELISA analytical biochemical technique of the MBS7225690 kit is based on RPS28 antibody-RPS28 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RPS28 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RPS28. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RPS28 elisa kit
Principle of the assay: RPS28 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPS28 antibody and an RPS28-HRP conjugate. The assay sample and buffer are incubated together with RPS28-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPS28 concentration since RPS28 from samples and RPS28-HRP conjugate compete for the anti-RPS28 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPS28 from the sample, fewer sites are left to bind RPS28-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPS28 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for RPS28 elisa kit

Typical Testing Data/Standard Curve (for reference only) of RPS28 elisa kit
RPS28 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7225690. Click to request current manual
NCBI/Uniprot data below describe general gene information for RPS28. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
7,841 Da
NCBI Official Full Name
40S ribosomal protein S28
NCBI Official Synonym Full Names
ribosomal protein S28
NCBI Official Symbol
RPS28  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
40S ribosomal protein S28
UniProt Protein Name
40S ribosomal protein S28
UniProt Gene Name
RPS28  [Similar Products]
UniProt Entry Name
RS28_HUMAN
NCBI Summary for RPS28
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S28E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Comments for RPS28
RPS28: Belongs to the ribosomal protein S28e family.

Protein type: Translation; RNA-binding; Ribosomal

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: small ribosomal subunit; cytoplasm; cytosol

Molecular Function: structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; ribosomal small subunit biogenesis and assembly; cellular protein metabolic process; translational elongation; translation; viral reproduction; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; translational termination; viral infectious cycle; rRNA processing

Disease: Diamond-blackfan Anemia 15 With Mandibulofacial Dysostosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with RPS28 elisa kit
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