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rpsG recombinant protein :: 30S ribosomal protein S7 Recombinant Protein

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Catalog # MBS717356
Unit / Price
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.05 mg  /  $190 +1 FREE 8GB USB
  0.1 mg  /  $285 +1 FREE 8GB USB
  0.2 mg  /  $460 +1 FREE 8GB USB
  0.5 mg  /  $750 +1 FREE 8GB USB
  1 mg  /  $1,180 +1 FREE 8GB USB
SDS-PAGE
Product Name

30S ribosomal protein S7 (rpsG), Recombinant Protein

Also Known As

Recombinant E Coli 30S ribosomal protein S7

Product Synonym Gene Name
b3341; JW3303[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
2-179aa; Full Length
Sequence Length
179
Sequence
PRRRVIGQRK ILPDPKFGSE LLAKFVNILM VDGKKSTAES IVYSALETLA QRSGKSELEA FEVALENVRP TVEVKSRRVG GSTYQVPVEV RPVRRNALAM RWIVEAARKR GDKSMALRLA NELSDAAENK GTAVKKREDV HRMAEANKAF AHYRWLSLRS FSHQAGASSK QPALGYLN
3D Structure
ModBase 3D Structure for P02359
Host
E Coli
Purity/Purification
Greater than 90% as determined by SDS-PAGE. (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of rpsG recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
rpsG recombinant protein
One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, where it has been shown to contact mRNA. Has been shown to contact tRNA in both the P and E sites; it probably blocks exit of the E site tRNA.

rpsG recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
NCBI/Uniprot data below describe general gene information for rpsG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
24kD
NCBI Official Full Name
30S ribosomal subunit protein S7
NCBI Official Symbol
NCBI Official Synonym Symbols
ECK3328; JW3303
  [Similar Products]
NCBI Protein Information
30S ribosomal subunit protein S7
UniProt Protein Name
30S ribosomal protein S7
Protein Family
UniProt Gene Name
UniProt Entry Name
RS7_ECOLI
NCBI Summary for rpsG
HT_Cmplx17_Mem: [More information is available at EcoGene: EG10906]. The S7 protein is a component of the 30S subunit of the ribosome. [More information is available at EcoCyc: EG10906].
UniProt Comments for rpsG
One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, where it has been shown to contact mRNA. Has been shown to contact tRNA in both the P and E sites; it probably blocks exit of the E site tRNA.
Product References and Citations for rpsG recombinant protein
The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) The primary structure of ribosomal protein S7 from E. coli strains K and B.Reinbolt J., Tritsch D., Wittmann-Liebold B.Biochimie 61:501-522(1979) DNA sequences from the str operon of Escherichia coli.Post L.E., Nomura M.J. Biol. Chem. 255:4660-4666(1980) Mutant sequences in the rpsL gene of Escherichia coli B/r mechanistic implications for spontaneous and ultraviolet light mutagenesis.Timms A.R., Steingrimsdottir H., Lehmann A.R., Bridges B.A.Mol. Gen. Genet. 232:89-96(1992) Comparison of the complete sequence of the str operon in Salmonella typhimurium and Escherichia coli.Johanson U., Hughes D.Gene 120:93-98(1992) Weigel C.T.O. Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies.Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.EMBO J. 14:4578-4588(1995) Photochemical cross-linking of initiation factor-3 to Escherichia coli 30 S ribosomal subunits.MacKeen L.A., Kahan L., Wahba A.J., Schwartz I.J. Biol. Chem. 255:10526-10531(1980) Direct cross-links between initiation factors 1, 2, and 3 and ribosomal proteins promoted by 2-iminothiolane.Boileau G., Butler P., Hershey J.W.B., Traut R.R.Biochemistry 22:3162-3170(1983) Assembly of the 30S subunit from Escherichia coli ribosomes occurs via two assembly domains which are initiated by S4 and S7.Nowotny V., Nierhaus K.H.Biochemistry 27:7051-7055(1988) Post-transcriptional regulation of the str operon in Escherichia coli. Ribosomal protein S7 inhibits coupled translation of S7 but not its independent translation.Saito K., Mattheakis L.C., Nomura M.J. Mol. Biol. 235:111-124(1994) The ribosomal neighbourhood of the central fold of tRNA cross-links from position 47 of tRNA located at the A, P or E site.Osswald M., Doering T., Brimacombe R.Nucleic Acids Res. 23:4635-4641(1995) The cross-link from the upstream region of mRNA to ribosomal protein S7 is located in the C-terminal peptide experimental verification of a prediction from modeling studies.Greuer B., Thiede B., Brimacombe R.RNA 5:1521-1525(1999) Tagging ribosomal protein S7 allows rapid identification of mutants defective in assembly and function of 30S subunits.Fredrick K., Dunny G.M., Noller H.F.J. Mol. Biol. 298:379-394(2000) Ribosomal protein S7 from Escherichia coli uses the same determinants to bind 16S ribosomal RNA and its messenger RNA.Robert F., Brakier-Gingras L.Nucleic Acids Res. 29:677-682(2001) Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry.Arnold R.J., Reilly J.P.Anal. Biochem. 269:105-112(1999) Interaction of translation initiation factor 3 with the 30S ribosomal subunit.Dallas A., Noller H.F.Mol. Cell 8:855-864(2001) All-atom homology model of the Escherichia coli 30S ribosomal subunit.Tung C.-S., Joseph S., Sanbonmatsu K.Y.Nat. Struct. Biol. 9:750-755(2002) Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement.Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.Cell 113:789-801(2003) Structures of the bacterial ribosome at 3.5 A resolution.Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D.Science 310:827-834(2005)

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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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