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RSAD2 elisa kit :: Human Virus Inhibitory Protein (Viperin) ELISA Kit

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Catalog # MBS2023571 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
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 Typical Testing Data/Standard Curve (for reference only)
Product Name

Virus Inhibitory Protein (Viperin) (RSAD2), ELISA Kit

Popular Item
Full Product Name

Virus Inhibitory Protein (Viperin) ELISA Kit

Product Synonym Names
Cig5; RSAD2; vig1; Radical S-Adenosyl Methionine Domain Containing 2; Viperin,Endoplasmic Reticulum-Associated,Interferon-Inducible; Cytomegalovirus-induced gene 5
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
607810
3D Structure
ModBase 3D Structure for Q8WXG1
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Viperin. No significant cross-reactivity or interference between Viperin and analogues was observed.
Samples
Tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
<0.055ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Viperin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Viperin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RSAD2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for RSAD2purchase
MBS2023571 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Virus Inhibitory Protein (Viperin) (RSAD2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RSAD2. The ELISA analytical biochemical technique of the MBS2023571 kit is based on RSAD2 antibody-RSAD2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RSAD2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RSAD2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RSAD2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Viperin in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to Viperin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Viperin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Viperin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Viperin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for RSAD2 elisa kit

Typical Testing Data/Standard Curve (for reference only) of RSAD2 elisa kit
RSAD2 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2023571. Click to request current manual
NCBI/Uniprot data below describe general gene information for RSAD2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,170 Da[Similar Products]
NCBI Official Full Name
viperin
NCBI Official Synonym Full Names
radical S-adenosyl methionine domain containing 2
NCBI Official Symbol
RSAD2  [Similar Products]
NCBI Official Synonym Symbols
cig5; vig1; cig33; 2510004L01Rik
  [Similar Products]
NCBI Protein Information
radical S-adenosyl methionine domain-containing protein 2
UniProt Protein Name
Radical S-adenosyl methionine domain-containing protein 2
UniProt Synonym Protein Names
Cytomegalovirus-induced gene 5 protein; Viperin; Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible
UniProt Gene Name
RSAD2  [Similar Products]
UniProt Entry Name
RSAD2_HUMAN
UniProt Comments for RSAD2
RSAD2: Interferon-inducible iron-sulfur (4FE-4S) cluster- binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), and human immunodeficiency virus (HIV-1). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphospate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63'-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins. Belongs to the radical SAM superfamily. RSAD2 family.

Chromosomal Location of Human Ortholog: 2p25.2

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; mitochondrial inner membrane; lipid particle

Molecular Function: protein binding; protein self-association; 4 iron, 4 sulfur cluster binding; metal ion binding; catalytic activity

Biological Process: positive regulation of toll-like receptor 7 signaling pathway; negative regulation of protein secretion; ossification; viral reproduction; negative regulation of viral genome replication; CD4-positive, alpha beta T cell differentiation; metabolic process; cytokine and chemokine mediated signaling pathway; response to virus; positive regulation of toll-like receptor 9 signaling pathway; regulation of ossification; defense response to virus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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