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rsrIM recombinant protein :: Rhodobacter sphaeroides Modification methylase RsrI Recombinant Protein

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Catalog # MBS1187431
Unit / Price
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  0.01 mg (E-Coli)  /  $235 +1 FREE 8GB USB
  0.05 mg (E-Coli)  /  $320 +1 FREE 8GB USB
  0.1 mg (E-Coli)  /  $535 +1 FREE 8GB USB
  0.2 mg (E-Coli)  /  $855 +1 FREE 8GB USB
  0.5 mg (E-Coli)  /  $1,125 +1 FREE 8GB USB
  1 mg (E-Coli)  /  $1,725 +2 FREE 8GB USB
SDS-PAGE
Product Name

Rhodobacter sphaeroides Modification methylase RsrI (rsrIM), Recombinant Protein

Popular Item
Full Product Name

Recombinant Rhodobacter sphaeroides Modification methylase RsrI

Product Synonym Names
Adenine-specific methyltransferase RsrI
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-319aa; Full Length
Sequence
MANRSHHNAG HRAMNALRKS GQKHSSESQL GSSEIGTTRH VYDVCDCLDT LAKLPDDSVQ LIICDPPYNI MLADWDDHMD YIGWAKRWLA EAERVLSPTG SIAIFGGLQY QGEAGSGDLI SIISHMRQNS KMLLANLIIW NYPNGMSAQR FFANRHEEIA WFAKTKKYFF DLDAVREPYD EETKAAYMKD KRLNPESVEK GRNPTNVWRM SRLNGNSLER VGHPTQKPAA VIERLVRALS HPGSTVLDFF AGSGVTARVA IQEGRNSICT DAAPVFKEYY QKQLTFLQDD GLIDKARSYE IVEGAANFGA ALQRGDVAS
3D Structure
ModBase 3D Structure for P14751
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater than 90% as determined by SDS-PAGE. (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of rsrIM recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
rsrIM recombinant protein
This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-? on both strands, and protects the DNA from cleavage by the RsrI endonuclease.

rsrIM recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
NCBI/Uniprot data below describe general gene information for rsrIM. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
51.6kD
NCBI Official Full Name
Modification methylase RsrI
UniProt Protein Name
Modification methylase RsrI
UniProt Synonym Protein Names
Adenine-specific methyltransferase RsrI
Protein Family
UniProt Gene Name
rsrIM  [Similar Products]
UniProt Synonym Gene Names
M.RsrI  [Similar Products]
UniProt Entry Name
MTR1_RHOSH
UniProt Comments for rsrIM
This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-? on both strands, and protects the DNA from cleavage by the RsrI endonuclease.
Product References and Citations for rsrIM recombinant protein
Purification, cloning and sequence analysis of RsrI DNA methyltransferase lack of homology between two enzymes, RsrI and EcoRI, that methylate the same nucleotide in identical recognition sequences.Kaszubska W., Aiken C., O'Connor D., Gumport R.I., Stephenson F.H., Greene P.J.Nucleic Acids Res. 17:10403-10425(1989) Nucleotide sequence of the gene encoding the RsrI methyltransferase.Stephenson F.H., Greene P.J.Nucleic Acids Res. 17:10503-10503(1989) Structure of RsrI methyltransferase, a member of the N6-adenine beta class of DNA methyltransferases.Scavetta R.D., Thomas C.B., Walsh M.A., Szegedi S., Joachimiak A., Gumport R.I., Churchill M.E.Nucleic Acids Res. 28:3950-3961(2000)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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