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RUNX2 elisa kit :: Human Runt-related transcription factor 2 ELISA Kit

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Catalog # MBS766006
Unit / Price
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  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Runt-related transcription factor 2 (RUNX2), ELISA Kit

Popular Item
Full Product Name

Human Runt-related transcription factor 2 ELISA Kit

Product Synonym Names
RUNX2/CBFA1/Acute myeloid leukemia 3 protein/AML3CCD/Core-binding factor subunit alpha-1/PEA2-alpha A/PEBP2A/PEBP2A1CCD1/PEBP2aA1/PEBP2-alpha A/runt domain; alpha subunit 1/runt-related transcription factor 2/SL3,AKV core-binding factor alpha A subunit/SL3-3 enhancer factor 1 alpha A subunit
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS766006 QC Report
OMIM
119600
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of RUNX2. No significant cross-reactivity or interference between RUNX2 and analogues was observed.
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RUNX2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RUNX2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RUNX2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for RUNX2purchase
MBS766006 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Runt-related transcription factor 2 (RUNX2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RUNX2. The ELISA analytical biochemical technique of the MBS766006 kit is based on RUNX2 antibody-RUNX2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RUNX2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RUNX2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RUNX2 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- RUNX2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- RUNX2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RUNX2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RUNX2 can be calculated.

Typical Testing Data/Standard Curve (for reference only) of RUNX2 elisa kit
RUNX2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS766006. Click to request current manual
NCBI/Uniprot data below describe general gene information for RUNX2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
54,249 Da
NCBI Official Full Name
runt-related transcription factor 2 isoform a
NCBI Official Synonym Full Names
runt related transcription factor 2
NCBI Official Symbol
RUNX2  [Similar Products]
NCBI Official Synonym Symbols
CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1
  [Similar Products]
NCBI Protein Information
runt-related transcription factor 2
UniProt Protein Name
Runt-related transcription factor 2
UniProt Synonym Protein Names
Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit
UniProt Gene Name
RUNX2  [Similar Products]
UniProt Synonym Gene Names
AML3; CBFA1; OSF2; PEBP2A; CBF-alpha-1; OSF-2; PEA2-alpha A; PEBP2-alpha A  [Similar Products]
UniProt Entry Name
RUNX2_HUMAN
NCBI Summary for RUNX2
This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2008]
UniProt Comments for RUNX2
AML3: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors. Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 and HIPK3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with KAT6A and KAT6B. Binds to cyclin B1 CCNB1. Interacts with DDX5. Specifically expressed in osteoblasts. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; transcription factor complex

Molecular Function: ATP binding; bHLH transcription factor binding; chromatin binding; histone deacetylase binding; protein binding; protein domain specific binding; transcription factor activity

Biological Process: BMP signaling pathway; cell maturation; chondrocyte development; chondrocyte differentiation; embryonic cranial skeleton morphogenesis; embryonic forelimb morphogenesis; endochondral ossification; gene expression; hemopoiesis; negative regulation of smoothened signaling pathway; negative regulation of transcription, DNA-dependent; neuron differentiation; odontogenesis of dentine-containing teeth; ossification; osteoblast development; osteoblast differentiation; osteoblast fate commitment; positive regulation of cell proliferation; positive regulation of chondrocyte differentiation; positive regulation of osteoblast differentiation; positive regulation of transcription, DNA-dependent; regulation of cell differentiation; regulation of fibroblast growth factor receptor signaling pathway; regulation of odontogenesis of dentine-containing teeth; regulation of transcription from RNA polymerase II promoter; stem cell differentiation; T cell differentiation; transcription initiation from RNA polymerase II promoter

Disease: Cleidocranial Dysplasia; Metaphyseal Dysplasia With Maxillary Hypoplasia With Or Without Brachydactyly
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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