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RUNX2 elisa kit :: Mouse runt-related transcription factor 2 ELISA Kit

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Catalog # MBS934869
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Typical Testing Data/Standard Curve (for reference only)
Product Name

runt-related transcription factor 2 (RUNX2), ELISA Kit

Popular Item
Full Product Name

Mouse Runt-related transcription factor 2, RUNX2 ELISA Kit

Product Synonym Names
Mouse Runt-related transcription factor 2 (RUNX2) ELISA kit; RP1-166H4.1; AML3; CBFA1; CCD; CCD1; MGC120022; MGC120023; OSF2; PEA2aA; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1; CBF-alpha 1; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A; runt-related transcription factor 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS934869 Testing Data
3D Structure
ModBase 3D Structure for Q08775
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse RUNX2. No significant cross-reactivity or interference between mouse RUNX2 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
3.12 ng/ml -200 ng/ml.
Sensitivity
The minimum detectable dose of mouse RUNX2 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of RUNX2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for RUNX2purchase
MBS934869 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the runt-related transcription factor 2 (RUNX2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RUNX2. The ELISA analytical biochemical technique of the MBS934869 kit is based on RUNX2 antibody-RUNX2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RUNX2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RUNX2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
RUNX2 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RUNX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RUNX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RUNX2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RUNX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of RUNX2 elisa kit
RUNX2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS934869. Click to request current manual
NCBI/Uniprot data below describe general gene information for RUNX2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
66,205 Da
NCBI Official Full Name
runt-related transcription factor 2 isoform 1
NCBI Official Synonym Full Names
runt related transcription factor 2
NCBI Official Symbol
Runx2  [Similar Products]
NCBI Official Synonym Symbols
Cbf; LS3; AML3; Osf2; Cbfa1; Cbfa-1; PEBP2aA; Pebp2a1; Pebpa2a
  [Similar Products]
NCBI Protein Information
runt-related transcription factor 2; CBF-alpha-1; PEA2-alpha A; PEBP2 alpha A; AKV core binding factor; core binding factor alpha 1; runt domain, alpha subunit 1; acute myeloid leukemia 3 protein; SL3-3 enhancer factor 1 alpha A subunit; osteoblast-specific transcription factor 2; polyomavirus enhancer-binding protein 2 alpha A subunit
UniProt Protein Name
Runt-related transcription factor 2
UniProt Synonym Protein Names
Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit
UniProt Gene Name
Runx2  [Similar Products]
UniProt Synonym Gene Names
Aml3; Cbfa1; Osf2; Pebp2a; CBF-alpha-1; OSF-2; PEA2-alpha A; PEBP2-alpha A  [Similar Products]
UniProt Entry Name
RUNX2_MOUSE
UniProt Comments for RUNX2
AML3: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors. Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 and HIPK3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with KAT6A and KAT6B. Binds to cyclin B1 CCNB1. Interacts with DDX5. Specifically expressed in osteoblasts. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; nucleus

Molecular Function: protein domain specific binding; protein binding; DNA binding; histone deacetylase binding; bHLH transcription factor binding; chromatin binding; transcription factor binding; transcription factor activity; ATP binding

Biological Process: transcription from RNA polymerase II promoter; embryonic forelimb morphogenesis; positive regulation of transcription, DNA-dependent; cell maturation; neuron differentiation; BMP signaling pathway; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; chondrocyte differentiation; skeletal development; regulation of odontogenesis of dentine-containing teeth; T cell differentiation; endochondral ossification; regulation of osteoblast differentiation; transcription, DNA-dependent; regulation of fibroblast growth factor receptor signaling pathway; embryonic cranial skeleton morphogenesis; stem cell differentiation; chondrocyte development; positive regulation of ossification; odontogenesis of dentine-containing teeth; osteoblast development; osteoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of chondrocyte differentiation; skeletal morphogenesis; negative regulation of smoothened signaling pathway; positive regulation of transcription from RNA polymerase II promoter; regulation of ossification; negative regulation of transcription, DNA-dependent; osteoblast fate commitment
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with RUNX2 elisa kit
Organs/Tissues associated with RUNX2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Epididymis Antibodies  >1 publications with RUNX2 and Epididymis
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