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S recombinant protein :: Large envelope protein (S) Recombinant Protein

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Catalog # MBS1211330
Unit / Price Please Inquire
SDS-PAGE
Product Name

Large envelope protein (S), Recombinant Protein

Popular Item
Also Known As

Recombinant Hepatitis B virus genotype B2 Large envelope protein (S)

Product Synonym Names
Recombinant Large envelope protein (S); Large envelope protein; L glycoprotein L-HBsAg; LHB Large S protein Large surface protein Major surface antigen
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
2-389
Sequence Length
389
Sequence
GTNLSVPNPL GFFPDHQLDP AFKANSDNPD WDLNPHKDNW PDSNKVGVGA FGPGFTPPHG GLLGWSPQAQ GILTTVPTAP PPASTNRQLG RKPTPLSPPL RDTHPQAMQW NSTTFHQTLQ DPRVRALYFP AGGSSSGTVN PVQNTASSIS SILSTTGDPV PNMENIASGL LGPLLVLQAG FFSLTKILTI PLSLDSWWTS LNFLGETPVC LGQNSQSQIS SHSPTCCPPI CPGYRWMCLR RFIIFLCILL LCLIFLLVLL DYQGMLPVCP LIPGSSTTST GPCKTCTTPA QGTSMFPSCC CTKPTDGNCT CIPIPSSWAF AKYLWEWASV RFSWLSLLVP FVQWFVGLSP TVWLSVIWMM WFWGPSLYNI LSPFMPLLPI FFCLWVYI
3D Structure
ModBase 3D Structure for P17397
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>=90%(SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Hepatitis B virus genotype B2 (isolate Indonesia/pIDW420/1988) (HBV-B)
Preparation and Storage
Store at -20 degree C. For extended storage, store at -20 or -80 degree C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 Degree C for up to one week.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of S recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
S recombinant protein
The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assumes fusion between virion membrane and endosomal membrane (Probable). In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein (By similarity).

S recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
NCBI/Uniprot data below describe general gene information for S. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
NCBI Official Full Name
Large envelope protein
UniProt Protein Name
Large envelope protein
UniProt Synonym Protein Names
L glycoprotein; L-HBsAg; LHB; Large S protein; Large surface protein; Major surface antigen
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
HBSAG_HBVB2
UniProt Comments for S
Function: The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assumes fusion between virion membrane and endosomal membrane

Probable. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein

By similarity.The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid

By similarity.

Subunit structure: Li-HBsAg interacts with capsid protein and with HDV Large delta antigen. Isoform M associates with host chaperone CANX through its pre-S2 N glycan. This association may be essential for M proper secretion

By similarity.

Subcellular location: Virion membrane

By similarity.

Domain: The large envelope protein is synthesized with the pre-S region at the cytosolic side of the endoplasmic reticulum and, hence will be within the virion after budding. Therefore the pre-S region is not N-glycosylated. Later a post-translational translocation of N-terminal pre-S and TM1 domains occur in about 50% of proteins at the virion surface. These molecules change their topology by an unknown mechanism, resulting in exposure of pre-S region at virion surface. For isoform M in contrast, the pre-S2 region is translocated cotranslationally to the endoplasmic reticulum lumen and is N-glycosylated.

Post-translational modification: Isoform M is N-terminally acetylated at a ratio of 90%, and N-glycosylated at the pre-S2 region

By similarity.Myristoylated

By similarity.

Biotechnological use: Systematic vaccination of individuals at risk of exposure to the virus has been the main method of controlling the morbidity and mortality associated with hepatitis B. The first hepatitis B vaccine was manufactured by the purification and inactivation of HBsAg obtained from the plasma of chronic hepatitis B virus carriers. The vaccine is now produced by recombinant DNA techniques and expression of the S isoform in yeast cells. The pre-S region do not seem to induce strong enough antigenic response.

Sequence similarities: Belongs to the orthohepadnavirus major surface antigen family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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