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S100A2 elisa kit :: Human S100 calcium binding protein A2 ELISA Kit

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Catalog # MBS938477
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

S100 calcium binding protein A2 (S100A2), ELISA Kit

Popular Item
Also Known As

Human Protein S100-A2, S100A2 ELISA Kit

Product Synonym Names
Human Protein S100-A2 (S100A2) ELISA kit; RP11-49N14.8; CAN19; MGC111539; S100L; OTTHUMP00000032968; OTTHUMP00000032969; S100 calcium-binding protein A2; S100 calcium binding protein A2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS938477 Testing Data
Sequence Length
98
OMIM
176993
3D Structure
ModBase 3D Structure for P29034
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human S100A2. No significant cross-reactivity or interference between human S100A2 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates.
Assay Type
Sandwich
Detection Range
1.56 ng/ml -100 ng/ml.
Sensitivity
The minimum detectable dose of human S100A2 is typically less than 0.39 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of S100A2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forS100A2purchase
MBS938477 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the S100 calcium binding protein A2 (S100A2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing S100A2. The ELISA analytical biochemical technique of the MBS938477 kit is based on S100A2 antibody-S100A2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect S100A2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, S100A2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
S100A2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S100A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of S100A2 elisa kit
S100A2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS938477. Click to request current manual
NCBI/Uniprot data below describe general gene information for S100A2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,117 Da
NCBI Official Full Name
protein S100-A2
NCBI Official Synonym Full Names
S100 calcium binding protein A2
NCBI Official Symbol
S100A2  [Similar Products]
NCBI Official Synonym Symbols
CAN19; S100L
  [Similar Products]
NCBI Protein Information
protein S100-A2; S100 calcium-binding protein A2
UniProt Protein Name
Protein S100-A2
UniProt Synonym Protein Names
CAN19; Protein S-100L; S100 calcium-binding protein A2
Protein Family
UniProt Gene Name
S100A2  [Similar Products]
UniProt Synonym Gene Names
S100L  [Similar Products]
UniProt Entry Name
S10A2_HUMAN
NCBI Summary for S100A2
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may have a tumor suppressor function. Chromosomal rearrangements and altered expression of this gene have been implicated in breast cancer. [provided by RefSeq, Jul 2008]
UniProt Comments for S100A2
S100A2: May act as a modulator against excess calcium accumulation in normal human mammary epithelial cells. May also play a role in suppressing tumor cell growth. Belongs to the S-100 family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1q21

Molecular Function: identical protein binding; protein binding; calcium ion binding

Biological Process: endothelial cell migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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