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S100A9 elisa kit :: Mouse S100 Calcium Binding Protein A9 ELISA Kit

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Catalog # MBS762309
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

S100 Calcium Binding Protein A9 (S100A9), ELISA Kit

Popular Item
Also Known As

Mouse S100 Calcium Binding Protein A9 ELISA Kit

Product Synonym Names
S100A9/Calgranulin B/MRP-14/CAGBMigration inhibitory factor-related protein 14/calgranulin B/calgranulin-B/Calgranulin-B/Calprotectin L1H subunit/CFAGMRP-14,60B8AG/CGLB/L1AG/LIAG/MAC387/MIF/MRP14Leukocyte L1 complex heavy chain/NIF/P14/p14/protein S100-A9/S100 calcium binding protein A9/S100 calcium binding protein A9 (calgranulin B)/S100 calcium-binding protein A9/S100 calcium-binding protein A9 (calgranulin B)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS762309 COA
Sequence Length
114
OMIM
123886
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of S100A9. No significant cross-reactivity or interference between S100A9 and analogues was observed.
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100A9 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100A9 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of S100A9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forS100A9purchase
MBS762309 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the S100 Calcium Binding Protein A9 (S100A9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing S100A9. The ELISA analytical biochemical technique of the MBS762309 kit is based on S100A9 antibody-S100A9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect S100A9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, S100A9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
S100A9 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- S100A9 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- S100A9 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the S100A9 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of S100A9 can be calculated.

Typical Testing Data/Standard Curve (for reference only) of S100A9 elisa kit
S100A9 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS762309. Click to request current manual
NCBI/Uniprot data below describe general gene information for S100A9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
13,242 Da
NCBI Official Full Name
S100 calcium binding protein A9
NCBI Official Synonym Full Names
S100 calcium binding protein A9
NCBI Official Symbol
S100A9  [Similar Products]
NCBI Official Synonym Symbols
MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
  [Similar Products]
NCBI Protein Information
protein S100-A9
UniProt Protein Name
Protein S100-A9
UniProt Synonym Protein Names
Calgranulin-B; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain; Migration inhibitory factor-related protein 14; MRP-14; p14; S100 calcium-binding protein A9
Protein Family
UniProt Gene Name
S100A9  [Similar Products]
UniProt Synonym Gene Names
CAGB; CFAG; MRP14; MRP-14; p14  [Similar Products]
UniProt Entry Name
S10A9_HUMAN
NCBI Summary for S100A9
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. This antimicrobial protein exhibits antifungal and antibacterial activity. [provided by RefSeq, Nov 2014]
UniProt Comments for S100A9
S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoskeleton; cytosol; extracellular region; extracellular space; nucleus; plasma membrane

Molecular Function: antioxidant activity; arachidonic acid binding; calcium ion binding; microtubule binding; protein binding; RAGE receptor binding; signal transducer activity; zinc ion binding

Biological Process: actin cytoskeleton reorganization; activation of NF-kappaB transcription factor; astrocyte development; autophagy; caspase activation; cell-cell signaling; chemokine production; cytokine production; defense response to bacterium; defense response to fungus; inflammatory response; innate immune response; leukocyte migration during inflammatory response; neutrophil chemotaxis; positive regulation of blood coagulation; positive regulation of cell growth; positive regulation of inflammatory response; positive regulation of peptide secretion; regulation of cytoskeleton organization and biogenesis; regulation of integrin biosynthetic process; regulation of translation; sequestering of zinc ion; signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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