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S100beta elisa kit :: Mouse S100beta Protein ELISA Kit

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Catalog # MBS733146
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

S100beta Protein, ELISA Kit

Popular Item
Also Known As

Mouse S100beta Protein ELISA Kit

Product Synonym Names
Mouse S100b Protein ELISA Kit
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
176990
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
50-1000pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This S100B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse S100B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of S100beta elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forS100betapurchase
MBS733146 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the S100beta Protein, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing S100beta. The ELISA analytical biochemical technique of the MBS733146 kit is based on S100beta antibody-S100beta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect S100beta antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, S100beta. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
S100beta elisa kit
Principle of the Assay: S100B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-S100B antibody and an S100B-HRP conjugate. The assay sample and buffer are incubated together with S100B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S100B concentration since S100B from samples and S100B-HRP conjugate compete for the anti-S100B antibody binding site. Since the number of sites is limited, as more sites are occupied by S100B from the sample, fewer sites are left to bind S100B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100B concentration in each sample is interpolated from this standard curve.
Product Categories/Family for S100beta elisa kit

Typical Testing Data/Standard Curve (for reference only) of S100beta elisa kit
S100beta elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS733146. Click to request current manual
NCBI/Uniprot data below describe general gene information for S100beta. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
10,713 Da
NCBI Official Full Name
S100 protein beta subunit
NCBI Official Synonym Full Names
S100 calcium binding protein B
NCBI Official Symbol
S100B  [Similar Products]
NCBI Official Synonym Symbols
NEF; S100; S100-B; S100beta
  [Similar Products]
NCBI Protein Information
protein S100-B; S-100 protein subunit beta; S-100 calcium-binding protein, beta chain; S100 calcium-binding protein, beta (neural)
UniProt Protein Name
Protein S100-B
UniProt Synonym Protein Names
S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B
UniProt Gene Name
S100B  [Similar Products]
UniProt Entry Name
S100B_HUMAN
NCBI Summary for S100beta
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]
UniProt Comments for S100beta
S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: ruffle; extracellular space; cell soma; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; extracellular region; nucleus

Molecular Function: identical protein binding; RAGE receptor binding; protein binding; protein homodimerization activity; zinc ion binding; calcium ion binding; tau protein binding; calcium-dependent protein binding

Biological Process: central nervous system development; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; response to glucocorticoid stimulus; memory; regulation of cell shape; cell proliferation; learning and/or memory; axonogenesis; astrocyte differentiation; positive regulation of cell proliferation; response to methylmercury; innate immune response; regulation of neuronal synaptic plasticity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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