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S1PR3 elisa kit :: Human sphingosine-1-phosphate receptor 3 ELISA Kit

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Catalog # MBS705399
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Typical Testing Data/Standard Curve (for reference only)
Product Name

sphingosine-1-phosphate receptor 3 (S1PR3), ELISA Kit

Popular Item
Full Product Name

Human sphingosine 1-phosphate receptor type 3, S1P3 ELISA Kit

Product Synonym Names
Human sphingosine 1-phosphate receptor type 3 (S1P3) ELISA Kit; EDG-3; EDG3; FLJ37523; FLJ93220; LPB3; MGC71696; S1P3; G protein-coupled receptor; endothelial differentiation gene-3; S1P receptor EDG3; endothelial differentiation; sphingolipid G-protein-coupled re; sphingosine-1-phosphate receptor 3
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS705399 Testing Data
OMIM
601965
3D Structure
ModBase 3D Structure for Q99500
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human S1P3. No significant cross-reactivity or interference between human S1P3 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
23.5 pg/ml-1500 pg/ml.
Sensitivity
The minimum detectable dose of human S1P3 is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of S1PR3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for S1PR3purchase
MBS705399 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sphingosine-1-phosphate receptor 3 (S1PR3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing S1PR3. The ELISA analytical biochemical technique of the MBS705399 kit is based on S1PR3 antibody-S1PR3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect S1PR3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, S1PR3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
S1PR3 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S1P3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S1P3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S1P3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S1P3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of S1PR3 elisa kit
S1PR3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS705399. Click to request current manual
NCBI/Uniprot data below describe general gene information for S1PR3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,250 Da
NCBI Official Full Name
sphingosine 1-phosphate receptor 3
NCBI Official Synonym Full Names
sphingosine-1-phosphate receptor 3
NCBI Official Symbol
S1PR3  [Similar Products]
NCBI Official Synonym Symbols
EDG3; LPB3; S1P3; EDG-3
  [Similar Products]
NCBI Protein Information
sphingosine 1-phosphate receptor 3; S1P receptor 3; S1P receptor EDG3; S1P receptor Edg-3; sphingosine 1-phosphate receptor Edg-3; endothelial differentiation G-protein coupled receptor 3; G protein-coupled receptor, endothelial differentiation gene-3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3
UniProt Protein Name
Sphingosine 1-phosphate receptor 3
UniProt Synonym Protein Names
Endothelial differentiation G-protein coupled receptor 3; Sphingosine 1-phosphate receptor Edg-3; S1P receptor Edg-3
UniProt Gene Name
S1PR3  [Similar Products]
UniProt Synonym Gene Names
EDG3; S1P receptor 3; S1P3; S1P receptor Edg-3  [Similar Products]
UniProt Entry Name
S1PR3_HUMAN
NCBI Summary for S1PR3
This gene encodes a member of the EDG family of receptors, which are G protein-coupled receptors. This protein has been identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function. [provided by RefSeq, Jul 2008]
UniProt Comments for S1PR3
Function: Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.1 PublicationManual assertion based on experiment in:Ref.9
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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