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SAG elisa kit :: Mouse S Antigen ELISA Kit

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Catalog # MBS731541
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

S Antigen (SAG), ELISA Kit

Also Known As

Mouse S Antigen ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
613758
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This SAG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SAG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SAG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSAGpurchase
MBS731541 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the S Antigen (SAG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SAG. The ELISA analytical biochemical technique of the MBS731541 kit is based on SAG antibody-SAG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SAG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SAG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SAG elisa kit
Principle of the Assay: SAG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SAG antibody and an SAG-HRP conjugate. The assay sample and buffer are incubated together with SAG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SAG concentration since SAG from samples and SAG-HRP conjugate compete for the anti-SAG antibody binding site. Since the number of sites is limited, as more sites are occupied by SAG from the sample, fewer sites are left to bind SAG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SAG concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SAG elisa kit

Typical Testing Data/Standard Curve (for reference only) of SAG elisa kit
SAG elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS731541. Click to request current manual
NCBI/Uniprot data below describe general gene information for SAG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,120 Da
NCBI Official Full Name
S-arrestin
NCBI Official Synonym Full Names
S-antigen; retina and pineal gland (arrestin)
NCBI Official Symbol
NCBI Official Synonym Symbols
RP47; S-AG
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NCBI Protein Information
S-arrestin; arrestin 1; 48 kDa protein; rod photoreceptor arrestin; retinal S-antigen (48 KDa protein)
UniProt Protein Name
S-arrestin
UniProt Synonym Protein Names
48 kDa protein; Retinal S-antigen; S-AG; Rod photoreceptor arrestin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
ARRS_HUMAN
NCBI Summary for SAG
Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. S-arrestin, also known as S-antigen, is a major soluble photoreceptor protein that is involved in desensitization of the photoactivated transduction cascade. It is expressed in the retina and the pineal gland and inhibits coupling of rhodopsin to transducin in vitro. Additionally, S-arrestin is highly antigenic, and is capable of inducing experimental autoimmune uveoretinitis. Mutations in this gene have been associated with Oguchi disease, a rare autosomal recessive form of night blindness. [provided by RefSeq, Jul 2008]
UniProt Comments for SAG
SAG: Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated- phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase. Retina and pineal gland. Belongs to the arrestin family.

Protein type: G protein regulator, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: photoreceptor outer segment; photoreceptor inner segment; cytosol

Molecular Function: opsin binding; phosphoprotein binding; protein phosphatase inhibitor activity

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; cell surface receptor linked signal transduction; regulation of rhodopsin mediated signaling; visual perception; negative regulation of catalytic activity

Disease: Oguchi Disease 1; Retinitis Pigmentosa 47
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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