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SCF recombinant protein

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Catalog # MBS538024
Unit / Price
  0.01 mg  /  $310 +1 FREE 8GB USB
SCF recombinant protein
Product Name

SCF, Recombinant Protein

Popular Item
Also Known As

SCF protein (Mouse)

Product Synonym Names
c-Kit Ligand protein; Mast Cell Growth Factor protein; MGF protein; Steel Factor protein; Stem Cell Factor protein
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
37
Sequence
Region of SCF protein correspond ing to amino acids MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA.
OMIM
145250
Host
Mouse
Purity/Purification
> 98% pure
Form/Format
Lyophilized from 10mM Na3PO4, pH 6.5.
Concentration
10 ug per vial; lyophillized (lot specific)
Protein Type
Recombinant
Biological Significance
SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on Mouse cells, while Mouse and rat SCF are fully active on human cells.
Bioactivity
The ED50 as determined by the dose-dependent stimulation of the proliferation of the human TF-1 cells is 1 x 10^5 units/mg.
Endotoxin Levels
Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
Expression System
E Coli
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of SCF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SCF recombinant protein
Purified recombinant Mouse SCF protein
Product Categories/Family for SCF recombinant protein
Applications Tested/Suitable for SCF recombinant protein
User optimized
NCBI/Uniprot data below describe general gene information for SCF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18.3 kDa
NCBI Official Full Name
stem cell factor, partial
NCBI Official Synonym Full Names
KIT ligand
NCBI Official Symbol
KITLG  [Similar Products]
NCBI Official Synonym Symbols
SF; MGF; SCF; FPH2; FPHH; KL-1; Kitl; SHEP7
  [Similar Products]
NCBI Protein Information
kit ligand
UniProt Protein Name
Kit ligand
UniProt Synonym Protein Names
Mast cell growth factor; MGF
Protein Family
UniProt Gene Name
KITLG  [Similar Products]
UniProt Synonym Gene Names
MGF; SCF; MGF; SCF; sKITLG  [Similar Products]
UniProt Entry Name
SCF_HUMAN
NCBI Summary for SCF
This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for SCF
SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: extracellular space; cytoskeleton; cytoplasm; extracellular region; integral to membrane; plasma membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; stem cell factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; embryonic hemopoiesis; germ cell programmed cell death; male gonad development; signal transduction; cell proliferation; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of mast cell apoptosis; positive regulation of melanocyte differentiation; innate immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of Ras protein signal transduction; cell adhesion; neural crest cell migration; positive regulation of DNA replication

Disease: Hyperpigmentation, Familial Progressive, 2; Skin/hair/eye Pigmentation, Variation In, 7
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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