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SDC1 elisa kit :: Pig Syndecan-1/CD138 (SDC1) ELISA Kit

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Catalog # MBS923990
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Syndecan-1/CD138 (SDC1), ELISA Kit

Popular Item
Full Product Name

Pig Syndecan-1/CD138, SDC1 ELISA Kit

Product Synonym Names
Pig Syndecan-1/CD138 (SDC1) ELISA Kit; CD138; SDC; SYND1; syndecan; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan proteoglycan 1; Syndecan-1/CD138 (SDC1)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS923990 Testing Data
OMIM
186355
3D Structure
ModBase 3D Structure for P18827
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of pig SDC1. No significant cross-reactivity or interference between pig SDC1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
5 ng/ml -320 ng/ml
Sensitivity
Typically less than 1.25 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SDC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SDC1purchase
MBS923990 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Syndecan-1/CD138 (SDC1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SDC1. The ELISA analytical biochemical technique of the MBS923990 kit is based on SDC1 antibody-SDC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SDC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SDC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SDC1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SDC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDC1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of SDC1 elisa kit
SDC1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS923990. Click to request current manual
NCBI/Uniprot data below describe general gene information for SDC1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
32,462 Da
NCBI Official Full Name
syndecan-1
NCBI Official Synonym Full Names
syndecan 1
NCBI Official Symbol
NCBI Official Synonym Symbols
SDC; CD138; SYND1; syndecan
  [Similar Products]
NCBI Protein Information
syndecan-1; CD138 antigen; syndecan proteoglycan 1; heparan sulfate proteoglycan fibroblast growth factor receptor
UniProt Protein Name
Syndecan-1
UniProt Synonym Protein Names
CD_antigen: CD138
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
SDC; SYND1  [Similar Products]
UniProt Entry Name
SDC1_HUMAN
NCBI Summary for SDC1
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]
UniProt Comments for SDC1
syndecan-1: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Belongs to the syndecan proteoglycan family.

Protein type: Extracellular matrix; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p24.1

Cellular Component: lysosomal lumen; protein complex; focal adhesion; cell surface; Golgi lumen; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein C-terminus binding; protein binding; glycoprotein binding

Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; myoblast development; response to cAMP; wound healing; glycosaminoglycan metabolic process; striated muscle cell development; response to toxin; response to glucocorticoid stimulus; pathogenesis; lipoprotein metabolic process; Wnt receptor signaling pathway through beta-catenin; Sertoli cell development; odontogenesis; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; response to hydrogen peroxide; ureteric bud development; carbohydrate metabolic process; retinoid metabolic process; response to calcium ion; inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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