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Add To Compare List SDC4 elisa kit :: Human syndecan 4 ELISA Kit Catalog # MBS706777 Unit / Price 24-Strip-Wells (LIMIT 1) / $285 +1 FREE 8GB USB 48-Strip-Wells / $555 +1 FREE 8GB USB 96-Strip-Wells / $780 +1 FREE 8GB USB 5x96-Strip-Wells / $2,970 +3 FREE 8GB USB 10x96-Strip-Wells / $5,670 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name syndecan 4 (SDC4), ELISA Kit Popular Item Full Product Name Human syndecan 4, SDC4 ELISA Kit Product Synonym Names Human syndecan 4 (SDC4) ELISA kit; MGC22217; SYND4; amphiglycan; ryudocan amphiglycan; ryudocan core protein; syndecan 4 (amphiglycan; ryudocan) ; syndecan proteoglycan 4; syndecan 4 Product Gene Name SDC4 elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual MBS706777 Testing Data Testing Data PDF 3D Structure ModBase 3D Structure for O35988 Species Reactivity Human Specificity This assay has high sensitivity and excellent specificity for detection of human SDC4. No significant cross-reactivity or interference between human SDC4 and analogues was observed. Samples Serum, plasma, tissue homogenates, Cell lysates Assay Type Sandwich Detection Range 0.625 ng/ml -40 ng/ml. Sensitivity The minimum detectable dose of human SDC4 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Preparation and Storage Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions. ISO Certification Manufactured in an ISO 9001:2015 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of SDC4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for SDC4 purchase MBS706777 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the syndecan 4 (SDC4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SDC4. The ELISA analytical biochemical technique of the MBS706777 kit is based on SDC4 antibody-SDC4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SDC4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SDC4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for SDC4 elisa kit Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SDC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SDC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDC4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDC4 bound in the initial step. The color development is stopped and the intensity of the color is measured. Typical Testing Data/Standard Curve (for reference only) of SDC4 elisa kit TOP Sample Manual Insert of MBS706777. NCBI/Uniprot data below describe general gene information for SDC4. It may not necessarily be applicable to this product. NCBI GI # 6755442 NCBI GeneID 20971 NCBI Accession # NP_035651.1 [Other Products] NCBI GenBank Nucleotide # NM_011521.2 [Other Products] UniProt Primary Accession # O35988 [Other Products] UniProt Related Accession # O35988[Other Products] Molecular Weight 21,482 Da TOP NCBI Official Full Name syndecan-4 NCBI Official Synonym Full Names syndecan 4 NCBI Official Symbol Sdc4 [Similar Products] NCBI Official Synonym Symbols Synd4; AA959608; AW108331; ryudocan; syndecan-4 [Similar Products] NCBI Protein Information syndecan-4; ryudocan core protein UniProt Protein Name Syndecan-4 UniProt Synonym Protein Names Ryudocan core protein Protein Family Syndecan UniProt Gene Name Sdc4 [Similar Products] UniProt Synonym Gene Names SYND4 [Similar Products] UniProt Entry Name SDC4_MOUSE TOP TOP UniProt Comments for SDC4 Function: Cell surface proteoglycan that bears heparan sulfate. Research Articles on SDC4 1. Sdc4 is required for cell surface targeting of transglutaminase 2 and the development of kidney fibrosis in chronic kidney disease. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. 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