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SDC4 elisa kit :: Mouse SDC4 ELISA Kit

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Catalog # MBS2501152
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

SDC4, ELISA Kit

Popular Item
Full Product Name

Mouse SDC4 (Syndecan 4) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
600017
3D Structure
ModBase 3D Structure for P31431
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse SDC4. No significant cross-reactivity or interference between Mouse SDC4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse SDC4 and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SDC4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSDC4purchase
MBS2501152 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the SDC4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SDC4. The ELISA analytical biochemical technique of the MBS2501152 kit is based on SDC4 antibody-SDC4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SDC4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SDC4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SDC4 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SDC4 in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SDC4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse SDC4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SDC4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse SDC4. You can calculate the concentration of Mouse SDC4 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of SDC4 elisa kit
SDC4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2501152. Click to request current manual
NCBI/Uniprot data below describe general gene information for SDC4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,545 Da
NCBI Official Full Name
syndecan-4
NCBI Official Synonym Full Names
syndecan 4
NCBI Official Symbol
NCBI Official Synonym Symbols
SYND4
  [Similar Products]
NCBI Protein Information
syndecan-4; amphiglycan; ryudocan amphiglycan; ryudocan core protein; syndecan 4 (amphiglycan, ryudocan); syndecan proteoglycan 4
UniProt Protein Name
Syndecan-4
UniProt Synonym Protein Names
Amphiglycan; Ryudocan core protein
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
SYND4  [Similar Products]
UniProt Entry Name
SDC4_HUMAN
NCBI Summary for SDC4
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]
UniProt Comments for SDC4
syndecan-4: a heparan sulfate proteoglycan type I membrane protein that belongs to the syndecan proteoglycan family. Expressed as a homodimer. Promotes cell spreading in a beta(1) integrin-dependent fashion through PKC-alpha and Rho.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: lysosomal lumen; costamere; cell surface; focal adhesion; integral to plasma membrane; Golgi lumen; plasma membrane; lipid raft

Molecular Function: protein binding; protein kinase C binding; fibronectin binding

Biological Process: chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; phototransduction, visible light; extracellular matrix organization and biogenesis; glycosaminoglycan catabolic process; positive regulation of focal adhesion formation; glycosaminoglycan metabolic process; ureteric bud development; positive regulation of protein kinase activity; positive regulation of stress fiber formation; carbohydrate metabolic process; pathogenesis; retinoid metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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