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SDF-1alpha active protein :: SDF-1alpha / CXCL12 Active Protein

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Catalog # MBS506103
Unit / Price
Scan QR to view Datasheet
  0.002 mg  /  $140 +1 FREE 8GB USB
  0.01 mg  /  $225 +1 FREE 8GB USB
  0.1 mg  /  $450 +1 FREE 8GB USB
  0.5 mg  /  $1,730 +2 FREE 8GB USB
  1 mg  /  $2,825 +3 FREE 8GB USB
Product Name

SDF-1alpha / CXCL12, Active Protein

Popular Item
Full Product Name

Recombinant Human SDF-1alpha / CXCL12

Product Synonym Names
Human SDF-1 alpha (CXCL12) ; Stromal Cell-Derived Factor-1 alpha; CXCL12; PBSF
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
OMIM
L36033 mRNA
3D Structure
ModBase 3D Structure for P48061
Host
E Coli
Purity/Purification
Purity is typically 98% as determined by HPLC, reducing and non-reducing SDS-PAGE, UV spectroscopy at 280 nm
Form/Format
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing
0.1% Trifluoroacetic Acid (TFA)
Species
Human
Bioactivity Assay
bioactive protein but no acceptaince criteria limits established
Reconstitution
Sterile water at 0.1 mg/mL
Acceptance Criteria LAL (EUs/ug)
<=1.00
Monomer/Dimer
Monomer
Endotoxin Level (kinetic LAL analysis)
Typically less than 0.05 EUs/mug protein (50% confidence range)
Biological Activity
The activity is determined by its ability to chemoattract human peripheral T cells at 10 - 75 ng/mL. This corresponds to an expected specific activity of 1 x 105 units/mg.
Media Components
Luria Broth
Preparation and Storage
12 months from date of receipt when stored at -20 degree C to-80 degree C as supplied.
1 month when stored at 4 degree C after reconstituting as directed.
3 months when stored at -20 degree C to -80 degree C after reconstituting as directed.
Active Protein Note
Refer to the datasheet for activity details. For other activity applications, researchers should evaluate this protein for suitability in their model system.
Other Notes
Small volumes of SDF-1alpha active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SDF-1alpha active protein
Stromal cell-derived factor-1 alpha (SDF-1a), also called CXCL12a, is one of two SDF-1 splice variants made by a wide variety of cells upon stimulation by inflammatory cytokines such as TNF, IL-1, and LPS. SDF-1a signals through the G protein-coupled receptor CXCR4 to recruit activated leukocytes. Human and mouse SDF-1a share 99% sequence identity.

Biological Activity of SDF-1alpha active protein
SDF-1alpha active protein Biological Activity image
NCBI/Uniprot data below describe general gene information for SDF-1alpha. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Non-glycosylated protein, containing 68 amino acids, with a molecular weight of 8 kDa.
NCBI Official Full Name
stromal cell-derived factor 1 isoform beta
NCBI Official Synonym Full Names
C-X-C motif chemokine ligand 12
NCBI Official Symbol
CXCL12  [Similar Products]
NCBI Official Synonym Symbols
IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
  [Similar Products]
NCBI Protein Information
stromal cell-derived factor 1
UniProt Protein Name
Stromal cell-derived factor 1
UniProt Synonym Protein Names
C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH
UniProt Gene Name
CXCL12  [Similar Products]
UniProt Synonym Gene Names
SDF1; SDF1A; SDF1B; SDF-1; hSDF-1; IRH; hIRH; PBSF  [Similar Products]
NCBI Summary for SDF-1alpha
This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
UniProt Comments for SDF-1alpha
Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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