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SDF1 elisa kit :: Rat Stromal Cell Derived Factor 1 ELISA Kit

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Catalog # MBS722311
Unit / Price
  48-Strip-Wells-(Competitive)  /  $440 +1 FREE 8GB USB
  48-Strip-Wells-(Sandwich)  /  $440 +1 FREE 8GB USB
  96-Strip-Wells-(Competitive)  /  $640 +1 FREE 8GB USB
  96-Strip-Wells-(Sandwich)  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells-(Competitive)  /  $2,895 +3 FREE 8GB USB
  5x96-Strip-Wells-(Sandwich)  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells-(Competitive)  /  $5,415 +6 FREE 8GB USB
  10x96-Strip-Wells-(Sandwich)  /  $5,415 +6 FREE 8GB USB
SDF1 elisa kit
Product Name

Stromal Cell Derived Factor 1 (SDF1), ELISA Kit

Popular Item
Also Known As

Rat Stromal Cell Derived Factor 1 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sandwich ELISA Manual Insert
Sandwich ELISA PDF Manual
Request for Current Manual Insert
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of SDF-1. No significant cross-reactivity or interference between SDF-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SDF-1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SDF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSDF1purchase
MBS722311 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Stromal Cell Derived Factor 1 (SDF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SDF1. The ELISA analytical biochemical technique of the MBS722311 kit is based on SDF1 antibody-SDF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SDF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SDF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SDF1 elisa kit
Intended Uses: This SDF-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat SDF-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay||SDF-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SDF-1. Standards or samples are then added to the microtiter plate wells and SDF-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SDF-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SDF-1 are added to each well to "sandwich" the SDF-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SDF-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF-1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SDF1 elisa kit
NCBI/Uniprot data below describe general gene information for SDF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
stromal cell-derived factor 1
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 12
NCBI Official Symbol
CXCL12  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
stromal cell-derived factor 1; chemokine ligand 12b; stromal cell derived factor 1; stromal cell-derived factor-1 beta; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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