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SDF1a/CXCL12 elisa kit :: Pig Stromal cell derived factor 1a, SDF-1a ELISA Kit

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Catalog # MBS700184
Unit / Price
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $475 +1 FREE 8GB USB
  96-Strip-Wells  /  $675 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,140 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Stromal cell derived factor 1a, SDF-1a (SDF1a/CXCL12), ELISA Kit

Popular Item
Full Product Name

Pig Stromal cell derived factor 1a, SDF-1a ELISA Kit

Product Synonym Names
Pig Stromal cell derived factor 1a; SDF-1a ELISA Kit; Stromal cell derived factor 1a; SDF-1a
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS700184 Testing Data
OMIM
600835
3D Structure
ModBase 3D Structure for P48061
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of pig SDF-1? . No significant cross-reactivity or interference between pig SDF-1? and analogues was observed.
Samples
Serum, plasma, cell culture supernates, tissue homogenates and Cell lysates Cell lysates Cell lysates Cell lysates Cell lysates Cell lysates Cell lysates
Assay Type
Sandwich
Detection Range
0.312 ng/ml -20 ng/ml.
Sensitivity
The minimum detectable dose of pig SDF-1? is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SDF1a/CXCL12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SDF1a/CXCL12purchase
MBS700184 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Stromal cell derived factor 1a, SDF-1a (SDF1a/CXCL12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SDF1a/CXCL12. The ELISA analytical biochemical technique of the MBS700184 kit is based on SDF1a/CXCL12 antibody-SDF1a/CXCL12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SDF1a/CXCL12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SDF1a/CXCL12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SDF1a/CXCL12 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SDF-1? has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SDF-1? present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDF-1? is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDF-1? bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of SDF1a/CXCL12 elisa kit
SDF1a/CXCL12 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS700184. Click to request current manual
NCBI/Uniprot data below describe general gene information for SDF1a/CXCL12. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,004 Da
NCBI Official Full Name
stromal cell-derived factor 1a
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 12
NCBI Official Symbol
CXCL12  [Similar Products]
NCBI Official Synonym Symbols
IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
  [Similar Products]
NCBI Protein Information
stromal cell-derived factor 1; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor
UniProt Protein Name
Stromal cell-derived factor 1
UniProt Synonym Protein Names
C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor; PBSF
UniProt Gene Name
CXCL12  [Similar Products]
UniProt Synonym Gene Names
SDF1; SDF1A; SDF1B; SDF-1; hSDF-1; IRH; hIRH; PBSF  [Similar Products]
UniProt Entry Name
SDF1_HUMAN
NCBI Summary for SDF1a/CXCL12
This gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]
UniProt Comments for SDF1a/CXCL12
Function: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.6 PublicationsManual assertion based on experiment in:Ref.14
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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