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SDH elisa kit :: Rat Sorbitol Dehydrogenase (SDH) ELISA Kit

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Catalog # MBS2023295 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $270 +1 FREE 8GB USB
  48-Strip-Wells  /  $505 +1 FREE 8GB USB
  96-Strip-Wells  /  $685 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,775 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,035 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Sorbitol Dehydrogenase (SDH), ELISA Kit

Popular Item
Also Known As

Sorbitol Dehydrogenase (SDH) ELISA Kit

Product Synonym Names
SORD; L-iditol 2-dehydrogenase
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
100
OMIM
182500
3D Structure
ModBase 3D Structure for Q00796
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Sorbitol Dehydrogenase (SDH).
No significant cross-reactivity or interference between Sorbitol Dehydrogenase (SDH) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
Detection Range
1.56-100ng/mL
Sensitivity
< 0.59ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Sorbitol Dehydrogenase (SDH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Sorbitol Dehydrogenase (SDH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SDH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSDHpurchase
MBS2023295 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sorbitol Dehydrogenase (SDH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SDH. The ELISA analytical biochemical technique of the MBS2023295 kit is based on SDH antibody-SDH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SDH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SDH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SDH elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sorbitol Dehydrogenase (SDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sorbitol Dehydrogenase (SDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sorbitol Dehydrogenase (SDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sorbitol Dehydrogenase (SDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for SDH elisa kit

Typical Testing Data/Standard Curve (for reference only) of SDH elisa kit
SDH elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2023295. Click to request current manual
NCBI/Uniprot data below describe general gene information for SDH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
10,812 Da
NCBI Official Full Name
sorbitol dehydrogenase
NCBI Official Synonym Full Names
sorbitol dehydrogenase
NCBI Official Symbol
NCBI Official Synonym Symbols
SORD1; HEL-S-95n
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NCBI Protein Information
sorbitol dehydrogenase
UniProt Protein Name
Sorbitol dehydrogenase
UniProt Synonym Protein Names
L-iditol 2-dehydrogenase
Protein Family
UniProt Gene Name
UniProt Entry Name
DHSO_HUMAN
NCBI Summary for SDH
Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]
UniProt Comments for SDH
SORD: Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. Belongs to the zinc-containing alcohol dehydrogenase family.

Protein type: Carbohydrate Metabolism - fructose and mannose; Oxidoreductase; EC 1.1.1.14

Chromosomal Location of Human Ortholog: 15q15.3

Cellular Component: extracellular space; membrane; mitochondrial membrane

Molecular Function: zinc ion binding; L-iditol 2-dehydrogenase activity; carbohydrate binding; NAD binding

Biological Process: sperm motility; L-xylitol metabolic process; L-xylitol catabolic process; glucose metabolic process; fructose biosynthetic process; sorbitol catabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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