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Secondary Antibody

Secondary Antibody Definition

The binding of antibodies to target antigens can be visualized by either colorimetric or fluorescent methods. The methods for visualization involve either direct or indirect binding. Primary antibodies can be directly conjugated to fluorophores, enzymes or other substances that will aid in the visualization, such as GFP, biotin or horseradish peroxidase. However, when primary antibodies are directly conjugated, this limits the number of applications that they will be suitable for, as well as the sensitivity. For this reason, most researchers prefer to use conjugated secondary antibodies that will bind to the primary antibody of interest.

Displaying 1 to 9 (of 9 products) order by Products Name.
5 nm Colloidal Gold Conjugated Protein G
Applications:
Immunohistochemistry (IHC) Frozen/Paraffin, Immunocytochemistry (ICC), Electron Microscopy (EM)
 
10 nm Colloidal Gold Conjugated Protein G
Applications:
Immunohistochemistry (IHC) Frozen/Paraffin, Immunocytochemistry (ICC), Electron Microscopy (EM)
 
FITC Conjugated Protein G
Applications:
Immunohistochemistry (IHC) Frozen/Paraffin, Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
 
Cy3 Conjugated Avidin
Applications:
Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), Indirect fluorescence of (IHC-F), Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunohistochemistry (IHC)
    Cy3 Conjugated Goat Anti-Mouse IgG secondary antibody
    Host/Reactivities:
    Host: Goat
    Applications:
    Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC)
    • Immunohistochemistry (IHC)
    • Immunohistochemistry (IHC)
      Cy3 Conjugated Goat Anti-Rabbit IgG secondary antibody
      Host/Reactivities:
      Host: Goat
      Applications:
      Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC)
      • Immunohistochemistry (IHC)
      • Immunohistochemistry (IHC)
        Cy3 Conjugated Rabbit Anti-Goat IgG secondary antibody
        Host/Reactivities:
        Host: Rabbit
        Applications:
        Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC)
         
        Cy3 Conjugated Rabbit Anti-Human IgG secondary antibody
        Host/Reactivities:
        Host: Rabbit
        Applications:
        Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC)
         
        Displaying 1 to 9 (of 9 products)
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