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Secondary Antibody

Secondary Antibody Definition

The binding of antibodies to target antigens can be visualized by either colorimetric or fluorescent methods. The methods for visualization involve either direct or indirect binding. Primary antibodies can be directly conjugated to fluorophores, enzymes or other substances that will aid in the visualization, such as GFP, biotin or horseradish peroxidase. However, when primary antibodies are directly conjugated, this limits the number of applications that they will be suitable for, as well as the sensitivity. For this reason, most researchers prefer to use conjugated secondary antibodies that will bind to the primary antibody of interest.

Displaying 1 to 8 (of 8 products) order by Products Name.
unconjugated Goat Anti-mouse IgG secondary antibody
Host/Reactivities:
Host: Goat
Applications:
Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
 
unconjugated Goat Anti-rabbit IgG secondary antibody
Host/Reactivities:
Host: Goat
Applications:
Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
 
unconjugated Human IgG secondary antibody
Host/Reactivities:
Host: Human
Applications:
Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
 
unconjugated Rabbit Anti-goat IgG secondary antibody
Host/Reactivities:
Host: Rabbit
Applications:
Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
 
unconjugated Rabbit Anti-human IgG secondary antibody
Host/Reactivities:
Host: Rabbit
Applications:
Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
 
unconjugated Rabbit Anti-mouse IgG secondary antibody
Host/Reactivities:
Host: Rabbit
Applications:
Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
 
unconjugated Rabbit Anti-rat IgG secondary antibody
Host/Reactivities:
Host: Rabbit
Applications:
Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
 
unconjugated Rabbit IgG secondary antibody
Host/Reactivities:
Host: Rabbit
Applications:
Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
 
Displaying 1 to 8 (of 8 products)
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