Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List SELE elisa kit :: Human E-Selectin ELISA Kit Catalog # MBS762424 Unit / Price 48-Strip-Wells / $300 +1 FREE 8GB USB 96-Strip-Wells / $415 +1 FREE 8GB USB 5x96-Strip-Wells / $1,825 +2 FREE 8GB USB 10x96-Strip-Wells / $3,425 +4 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name E-Selectin, ELISA Kit Popular Item Full Product Name Human E-Selectin ELISA Kit Product Synonym Names E-Selectin/SELE/CD62E/CD62E/ELAM1/LECAM2/SELE/CD62 antigen-like family member E/CD62E/CD62E antigen/ELAM/ELAM-14 Product Gene Name SELE elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual Request for Current Manual Insert Request Current Manual MBS762424 QC Report QC Report PDF OMIM 131210 Species Reactivity Human Specificity This assay has high sensitivity and excellent specificity for detection of SELE . No significant cross-reactivity or interference between SELE and analogues was observed. Samples Serum, plasma and other biological fluids. Assay Type Sandwich ELISA, Double Antibody Detection Range 78.125-5000pg/ml Sensitivity 46.875pg/ml Intra-assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SELE were tested 20 times on one plate, respectively. Intra-Assay: CV<8% Inter-assay Precision Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SELE were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10% Preparation and Storage Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C). ISO Certification Manufactured in an ISO 9001:2008 Certified Laboratory. Product Note Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual. Other Notes Small volumes of SELE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms forSELEpurchase MBS762424 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the E-Selectin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SELE. The ELISA analytical biochemical technique of the MBS762424 kit is based on SELE antibody-SELE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SELE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SELE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for SELE elisa kit Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- SELE antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- SELE antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SELE amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SELE can be calculated. Typical Testing Data/Standard Curve (for reference only) of SELE elisa kit TOP Sample Manual Insert of MBS762424. Click to request current manual NCBI/Uniprot data below describe general gene information for SELE. It may not necessarily be applicable to this product. NCBI GI # 187960042 NCBI GeneID 6401 NCBI Accession # NP_000441.2 [Other Products] NCBI GenBank Nucleotide # NM_000450.2 [Other Products] UniProt Secondary Accession # P16111; A2RRD6[Other Products] UniProt Related Accession # P16581[Other Products] Molecular Weight 66,655 Da TOP NCBI Official Full Name E-selectin NCBI Official Synonym Full Names selectin E NCBI Official Symbol SELE [Similar Products] NCBI Official Synonym Symbols ELAM; ESEL; CD62E; ELAM1; LECAM2 [Similar Products] NCBI Protein Information E-selectin UniProt Protein Name E-selectin UniProt Synonym Protein Names CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD_antigen: CD62E Protein Family E-selectin UniProt Gene Name SELE [Similar Products] UniProt Synonym Gene Names ELAM1; ELAM-1; LECAM2 [Similar Products] UniProt Entry Name LYAM2_HUMAN TOP NCBI Summary for SELE The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008] TOP UniProt Comments for SELE SELE: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Belongs to the selectin/LECAM family.Protein type: Membrane protein, integralChromosomal Location of Human Ortholog: 1q22-q25Cellular Component: caveola; coated pit; cortical cytoskeleton; extracellular space; integral to membrane; lipid raft; perinuclear region of cytoplasm; plasma membraneMolecular Function: phospholipase binding; protein binding; sialic acid binding; transmembrane receptor activityBiological Process: actin filament-based process; blood coagulation; calcium-mediated signaling; heterophilic cell adhesion; inflammatory response; leukocyte adhesion; leukocyte migration; leukocyte migration during inflammatory response; leukocyte tethering or rolling; phospholipase C activation; positive regulation of leukocyte migration; positive regulation of receptor internalization; regulation of inflammatory response; response to lipopolysaccharideDisease: Hypertension, Essential Research Articles on SELE 1. Gene-based study revealed significant associations of the SELE gene with Blood Pressure phenotypes for the first time among the Chinese Han population. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated withSELE elisa kit Reference Product PubMed Publications JUN elisa kit >34 publications with SELE and JUN SELL elisa kit >16 publications with SELE and SELL NFKB1 elisa kit >4 publications with SELE and NFKB1 RELA elisa kit >3 publications with SELE and RELA CD44 elisa kit >2 publications with SELE and CD44 Pathways associated with SELE elisa kit Products by Pathway Pathway Diagram AGE-RAGE Signaling Pathway In Diabetic Complications antibodies AGE-RAGE Signaling Pathway In Diabetic Complications Diagram AGE-RAGE Signaling Pathway In Diabetic Complications antibodies AGE-RAGE Signaling Pathway In Diabetic Complications Diagram ATF-2 Transcription Factor Network Pathway antibodies ATF-2 Transcription Factor Network Pathway Diagram African Trypanosomiasis Pathway antibodies African Trypanosomiasis Pathway Diagram African Trypanosomiasis Pathway antibodies African Trypanosomiasis Pathway Diagram Cell Adhesion Molecules (CAMs) Pathway antibodies Cell Adhesion Molecules (CAMs) Pathway Diagram Cell Adhesion Molecules (CAMs) Pathway antibodies Cell Adhesion Molecules (CAMs) Pathway Diagram Cell Surface Interactions At The Vascular Wall Pathway antibodies Cell Surface Interactions At The Vascular Wall Pathway Diagram Glucocorticoid Receptor Regulatory Network Pathway antibodies Glucocorticoid Receptor Regulatory Network Pathway Diagram Hemostasis Pathway antibodies Hemostasis Pathway Diagram Diseases associated with SELE elisa kit Disease Name Pubmed Publications Inflammation Antibodies >102 publications with SELE and Inflammation Cardiovascular Diseases Antibodies >100 publications with SELE and Cardiovascular Diseases Necrosis Antibodies >50 publications with SELE and Necrosis Atherosclerosis Antibodies >41 publications with SELE and Atherosclerosis Lung Diseases Antibodies >27 publications with SELE and Lung Diseases Heart Diseases Antibodies >27 publications with SELE and Heart Diseases Myocardial Ischemia Antibodies >24 publications with SELE and Myocardial Ischemia Hypertension Antibodies >21 publications with SELE and Hypertension Insulin Resistance Antibodies >14 publications with SELE and Insulin Resistance Myocardial Infarction Antibodies >13 publications with SELE and Myocardial Infarction Organs/Tissues associated with SELE elisa kit Organ/Tissue Name Pubmed Publications Vascular Antibodies >141 publications with SELE and Vascular Heart Antibodies >26 publications with SELE and Heart Lung Antibodies >23 publications with SELE and Lung Brain Antibodies >12 publications with SELE and Brain Liver Antibodies >11 publications with SELE and Liver Muscle Antibodies >10 publications with SELE and Muscle Connective Tissue Antibodies >9 publications with SELE and Connective Tissue Testis Antibodies >6 publications with SELE and Testis Uterus Antibodies >6 publications with SELE and Uterus Placenta Antibodies >3 publications with SELE and Placenta TOP © 2006-18 MyBioSource.com. 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