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SELP elisa kit :: Human P-Selectin ELISA Kit

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Catalog # MBS176001
Unit / Price
  96-Strip-Wells  /  $360 +1 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

P-Selectin (SELP), ELISA Kit

Popular Item
Full Product Name

Human P-Selectin PicoKine ELISA Kit

Product Synonym Names
P-selectin; selectin P (granule membrane protein 140kDa, antigen CD62); Antigen CD62; CD62; CD62 antigen like family member P; CD62 antigen-like family member P; CD62P; FLJ45155; GMP 140; GMP-140; GMP140; Granule membrane protein 140; Granule membrane protein 140kDa; Granulocyte membrane protein; GRMP; LECAM3; Leukocyte endothelial cell adhesion molecule 3; Leukocyte-endothelial cell adhesion molecule 3; LYAM3_HUMAN; P selectin; P-selectin; PADGEM; Platelet activation dependent granule external membrane protein; Platelet activation dependent granule-external membrane protein; Platelet alpha granule membrane protein; PSEL; Selectin P; Selectin P (granule membrane protein 140kDa antigen CD62); SELP
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene+601367
3D Structure
ModBase 3D Structure for P16109
Species Reactivity
Specificity
Natural and recombinant Human P-Selectin
Assay Type
Quantitative Sandwich
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<5pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SELP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSELPpurchase
MBS176001 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the P-Selectin (SELP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SELP. The ELISA analytical biochemical technique of the MBS176001 kit is based on SELP antibody-SELP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SELP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SELP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SELP elisa kit
Principle of the Assay: Human P-Selectin ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for P-Selectin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: W42-A771) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human P-Selectin amount of sample captured in plate.

Background: P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1(PSGL-1). The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80 KDa.

Typical Testing Data/Standard Curve (for reference only) of SELP elisa kit
SELP elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS176001. Click to request current manual
NCBI/Uniprot data below describe general gene information for SELP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
90,834 Da
NCBI Official Full Name
P-selectin
NCBI Official Synonym Full Names
selectin P (granule membrane protein 140kDa, antigen CD62)
NCBI Official Symbol
NCBI Official Synonym Symbols
CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
  [Similar Products]
NCBI Protein Information
P-selectin; GMP-140; granule membrane protein 140; granulocyte membrane protein; CD62 antigen-like family member P; platelet alpha-granule membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein
UniProt Protein Name
P-selectin
UniProt Synonym Protein Names
CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD_antigen: CD62P
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GMRP; GRMP; GMP-140; LECAM3; PADGEM  [Similar Products]
UniProt Entry Name
LYAM3_HUMAN
NCBI Summary for SELP
This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]
UniProt Comments for SELP
SELP: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the selectin/LECAM family.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 1q22-q25

Cellular Component: nucleoplasm; extracellular space; platelet alpha granule membrane; integral to plasma membrane; cytoplasm; plasma membrane; platelet dense granule membrane; external side of plasma membrane

Molecular Function: heparin binding; protein binding; lipopolysaccharide binding; glycosphingolipid binding; glycoprotein binding; calcium-dependent protein binding; sialic acid binding; fucose binding

Biological Process: platelet activation; positive regulation of leukocyte migration; response to lipopolysaccharide; defense response to Gram-negative bacterium; heterophilic cell adhesion; positive regulation of phosphoinositide 3-kinase cascade; leukocyte adhesion; platelet degranulation; regulation of integrin activation; leukocyte tethering or rolling; blood coagulation; cell adhesion; inflammatory response; leukocyte migration

Disease: Ige Responsiveness, Atopic
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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