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SEMA3A elisa kit :: Human Semaphorin 3A (SEMA3A) ELISA Kit

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Catalog # MBS160764
Unit / Price
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  48-Strip-Wells  /  $260 +1 FREE 8GB USB
  96-Strip-Wells  /  $395 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,610 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,135 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Semaphorin 3A (SEMA3A), ELISA Kit

Popular Item
Full Product Name

Human Semaphorin 3A (SEMA3A) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q14563
Species Reactivity
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.3ng/ml - 90ng/ml
Sensitivity
0.14ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SEMA3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SEMA3Apurchase
MBS160764 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Semaphorin 3A (SEMA3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SEMA3A. The ELISA analytical biochemical technique of the MBS160764 kit is based on SEMA3A antibody-SEMA3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SEMA3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SEMA3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SEMA3A elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Semaphorin 3A (also known as Sema3A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human Sema3A antibody. Sema3A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human Sema3A Antibody is added and binds to Sema3A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Sema3A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human Sema3A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Typical Testing Data/Standard Curve (for reference only) of SEMA3A elisa kit
SEMA3A elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS160764. Click to request current manual
NCBI/Uniprot data below describe general gene information for SEMA3A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
88,889 Da
NCBI Official Full Name
semaphorin-3A
NCBI Official Synonym Full Names
semaphorin 3A
NCBI Official Symbol
SEMA3A  [Similar Products]
NCBI Official Synonym Symbols
HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III
  [Similar Products]
NCBI Protein Information
semaphorin-3A
UniProt Protein Name
Semaphorin-3A
UniProt Synonym Protein Names
Semaphorin III; Sema III
Protein Family
UniProt Gene Name
SEMA3A  [Similar Products]
UniProt Synonym Gene Names
SEMAD; Sema III  [Similar Products]
NCBI Summary for SEMA3A
This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]
UniProt Comments for SEMA3A
Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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