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SEMA3A elisa kit :: Human Semaphorin 3A (SEMA3A) ELISA Kit

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Catalog # MBS2020300 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $285 +1 FREE 8GB USB
  48-Strip-Wells  /  $535 +1 FREE 8GB USB
  96-Strip-Wells  /  $725 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,945 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,345 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Semaphorin 3A (SEMA3A), ELISA Kit

Popular Item
Also Known As

Semaphorin 3A (SEMA3A) ELISA Kit

Product Synonym Names
SEMAL; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SemD; Coll-1; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
771
OMIM
603961
3D Structure
ModBase 3D Structure for Q14563
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Semaphorin 3A (SEMA3A).
No significant cross-reactivity or interference between Semaphorin 3A (SEMA3A) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.057ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Semaphorin 3A (SEMA3A) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Semaphorin 3A (SEMA3A) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SEMA3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSEMA3Apurchase
MBS2020300 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Semaphorin 3A (SEMA3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SEMA3A. The ELISA analytical biochemical technique of the MBS2020300 kit is based on SEMA3A antibody-SEMA3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SEMA3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SEMA3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SEMA3A elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3A (SEMA3A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 3A (SEMA3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 3A (SEMA3A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Semaphorin 3A (SEMA3A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for SEMA3A elisa kit

Typical Testing Data/Standard Curve (for reference only) of SEMA3A elisa kit
SEMA3A elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2020300. Click to request current manual
NCBI/Uniprot data below describe general gene information for SEMA3A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
88,889 Da
NCBI Official Full Name
semaphorin-3A
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
NCBI Official Symbol
SEMA3A  [Similar Products]
NCBI Official Synonym Symbols
HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III
  [Similar Products]
NCBI Protein Information
semaphorin-3A
UniProt Protein Name
Semaphorin-3A
UniProt Synonym Protein Names
Semaphorin III; Sema III
Protein Family
UniProt Gene Name
SEMA3A  [Similar Products]
UniProt Synonym Gene Names
SEMAD; Sema III  [Similar Products]
UniProt Entry Name
SEM3A_HUMAN
NCBI Summary for SEMA3A
This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]
UniProt Comments for SEMA3A
SEMA3A: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. Belongs to the semaphorin family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p12.1

Cellular Component: membrane; axon; dendrite; extracellular region

Molecular Function: chemorepellent activity; receptor activity; semaphorin receptor binding

Biological Process: facial nerve structural organization; trigeminal nerve structural organization; axon guidance; apoptosis; regulation of heart rate; dendrite morphogenesis; olfactory bulb development; neuron migration; axonal fasciculation; negative chemotaxis; negative regulation of axon extension involved in axon guidance; axon extension involved in axon guidance; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; sympathetic nervous system development; branchiomotor neuron axon guidance; regulation of axon extension involved in axon guidance; nerve development; sensory system development

Disease: Hypogonadotropic Hypogonadism 16 With Or Without Anosmia
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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