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SEMA4A elisa kit :: Human sema domain, immunoglobulin domain (Ig) , transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A ELISA Kit

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Catalog # MBS940246
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

sema domain, immunoglobulin domain (Ig) , transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A (SEMA4A), ELISA Kit

Popular Item
Also Known As

Human Semaphorin-4A, SEMA4A ELISA Kit

Product Synonym Names
Human Semaphorin-4A (SEMA4A) ELISA kit; RP11-54H19.2; CORD10; FLJ12287; RP35; SEMAB; SEMB; OTTHUMP00000015917; OTTHUMP00000015918; sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; 4A; semaphori; sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; (semaphorin) 4A
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS940246 Testing Data
Sequence Length
761
OMIM
610283
3D Structure
ModBase 3D Structure for Q9H3S1
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human SEMA4A. No significant cross-reactivity or interference between human SEMA4A and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Detection Range
0.312 ng/ml -20 ng/mL
Sensitivity
<0.078 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SEMA4A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSEMA4Apurchase
MBS940246 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A (SEMA4A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SEMA4A. The ELISA analytical biochemical technique of the MBS940246 kit is based on SEMA4A antibody-SEMA4A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SEMA4A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SEMA4A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SEMA4A elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEMA4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA4A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA4A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of SEMA4A elisa kit
SEMA4A elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS940246. Click to request current manual
NCBI/Uniprot data below describe general gene information for SEMA4A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
83,574 Da
NCBI Official Full Name
semaphorin-4A isoform 1
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
NCBI Official Symbol
SEMA4A  [Similar Products]
NCBI Official Synonym Symbols
RP35; SEMB; SEMAB; CORD10
  [Similar Products]
NCBI Protein Information
semaphorin-4A; sema B; semaphorin-B
UniProt Protein Name
Semaphorin-4A
UniProt Synonym Protein Names
Semaphorin-B; Sema B
Protein Family
UniProt Gene Name
SEMA4A  [Similar Products]
UniProt Synonym Gene Names
SEMAB; SEMB; UNQ783/PRO1317; Sema B  [Similar Products]
UniProt Entry Name
SEM4A_HUMAN
NCBI Summary for SEMA4A
This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]
UniProt Comments for SEMA4A
SEMA4A: a single-pass type I membrane protein that inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Defects in SEMA4A are the cause of retinitis pigmentosa type 35 and cone-rod dystrophy type 10.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: plasma membrane; integral to membrane

Molecular Function: protein binding; receptor activity

Biological Process: axon guidance; regulation of cell shape; negative regulation of angiogenesis; T-helper 1 cell differentiation; angiogenesis

Disease: Retinitis Pigmentosa 35; Cone-rod Dystrophy 10
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withSEMA4A elisa kit
 Reference Product  PubMed Publications
 PLXND1 elisa kit  >1 publications with SEMA4A and PLXND1
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