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SEMA4C elisa kit :: Human SEMA4C ELISA Kit

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Catalog # MBS2504258
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

SEMA4C, ELISA Kit

Popular Item
Also Known As

Human SEMA4C (Semaphorin 4C) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
833
OMIM
604462
3D Structure
ModBase 3D Structure for Q9C0C4
Species Reactivity
Specificity
This kit recognizes natural and recombinant Human SEMA4C. No significant cross-reactivity or interference between Human SEMA4C and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SEMA4C elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSEMA4Cpurchase
MBS2504258 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the SEMA4C, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SEMA4C. The ELISA analytical biochemical technique of the MBS2504258 kit is based on SEMA4C antibody-SEMA4C antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SEMA4C antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SEMA4C. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SEMA4C elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human SEMA4C concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SEMA4C. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SEMA4C and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SEMA4C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SEMA4C. You can calculate the concentration of SEMA4C in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of SEMA4C elisa kit
SEMA4C elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2504258. Click to request current manual
NCBI/Uniprot data below describe general gene information for SEMA4C. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
92,623 Da
NCBI Official Full Name
semaphorin-4C
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C
NCBI Official Symbol
SEMA4C  [Similar Products]
NCBI Official Synonym Symbols
SEMAF; SEMAI; SEMACL1; M-SEMA-F
  [Similar Products]
NCBI Protein Information
semaphorin-4C; M-Sema F
UniProt Protein Name
Semaphorin-4C
Protein Family
UniProt Gene Name
SEMA4C  [Similar Products]
UniProt Synonym Gene Names
KIAA1739; SEMAI  [Similar Products]
UniProt Entry Name
SEM4C_HUMAN
UniProt Comments for SEMA4C
SEMA4C: Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration. Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade. Belongs to the semaphorin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: extracellular space; postsynaptic membrane; synaptic vesicle membrane; postsynaptic density; integral to membrane; cell junction

Molecular Function: receptor activity

Biological Process: cell migration in hindbrain; muscle cell differentiation; neural tube closure; cerebellum development; positive regulation of stress-activated MAPK cascade
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with SEMA4C elisa kit
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