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SEMA4D elisa kit :: Human Semaphorin 4D (SEMA4D) ELISA Kit

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Catalog # MBS2703296 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
  24-Strip-Wells  /  $210 +1 FREE 8GB USB
  48-Strip-Wells  /  $345 +1 FREE 8GB USB
  96-Strip-Wells  /  $460 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,890 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,510 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Semaphorin 4D (SEMA4D), ELISA Kit

Popular Item
Full Product Name

Human Semaphorin 4D (SEMA4D) ELISA Kit

Product Synonym Names
CD100; A8; BB18; GR3; M-Sema G; M-Sema-G; SEMAJ; Coll-4; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
U60800 mRNA
3D Structure
ModBase 3D Structure for Q92854
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Semaphorin 4D (SEMA4D).
No significant cross-reactivity or interference between Semaphorin 4D (SEMA4D) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue Homogenates, Cell Lysates and Other Biological Fluids
Detection Range
1.56-100ng/mL
Sensitivity
< 13.5pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Semaphorin 4D (SEMA4D) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Semaphorin 4D (SEMA4D) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SEMA4D elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSEMA4Dpurchase
MBS2703296 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Semaphorin 4D (SEMA4D) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SEMA4D. The ELISA analytical biochemical technique of the MBS2703296 kit is based on SEMA4D antibody-SEMA4D antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SEMA4D antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SEMA4D. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SEMA4D elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 4D (SEMA4D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 4D (SEMA4D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 4D (SEMA4D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Semaphorin 4D (SEMA4D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of SEMA4D elisa kit
SEMA4D elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2703296. Click to request current manual
NCBI/Uniprot data below describe general gene information for SEMA4D. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
82,164 Da
NCBI Official Full Name
semaphorin-4D isoform 1
NCBI Official Synonym Full Names
semaphorin 4D
NCBI Official Symbol
SEMA4D  [Similar Products]
NCBI Official Synonym Symbols
CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G
  [Similar Products]
NCBI Protein Information
semaphorin-4D
UniProt Protein Name
Semaphorin-4D
UniProt Synonym Protein Names
A8; BB18; GR3; CD_antigen: CD100
Protein Family
UniProt Gene Name
SEMA4D  [Similar Products]
UniProt Synonym Gene Names
C9orf164; CD100; SEMAJ  [Similar Products]
UniProt Comments for SEMA4D
SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: extracellular space; integral component of plasma membrane; plasma membrane

Molecular Function: protein binding; receptor activity; receptor binding; semaphorin receptor binding; transmembrane receptor activity

Biological Process: cell adhesion; immune response; negative chemotaxis; negative regulation of apoptosis; negative regulation of cell adhesion; negative regulation of osteoblast differentiation; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell migration; positive regulation of collateral sprouting; positive regulation of GTPase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein phosphorylation; regulation of cell projection organization; regulation of cell shape; regulation of dendrite morphogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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