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SEPP1 elisa kit :: Mouse SEPP1 ELISA Kit

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Catalog # MBS2500989
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

SEPP1, ELISA Kit

Popular Item
Also Known As

Mouse SEPP1 (Selenoprotein P) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2500989 COA
Sequence Length
411
OMIM
601484
3D Structure
ModBase 3D Structure for P49908
Species Reactivity
Specificity
This kit recognizes natural and recombinant Mouse SEPP1. No significant cross-reactivity or interference between Mouse SEPP1 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
125-8000pg/mL
Sensitivity
Min: 75pg/mL; Max: 8000pg/mL
Intended Uses
This ELISA kit applies to the in vitro quantitative determination of Mouse SEPP1 concentrations in serum, plasma and other biological fluids.
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SEPP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forSEPP1purchase
MBS2500989 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the SEPP1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SEPP1. The ELISA analytical biochemical technique of the MBS2500989 kit is based on SEPP1 antibody-SEPP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SEPP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SEPP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SEPP1 elisa kit
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SEPP1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SEPP1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SEPP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SEPP1. You can calculate the concentration of SEPP1 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of SEPP1 elisa kit
SEPP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2500989. Click to request current manual
NCBI/Uniprot data below describe general gene information for SEPP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
43,174 Da
NCBI Official Full Name
selenoprotein P isoform 2
NCBI Official Synonym Full Names
selenoprotein P, plasma, 1
NCBI Official Symbol
SEPP1  [Similar Products]
NCBI Official Synonym Symbols
SeP; SELP
  [Similar Products]
NCBI Protein Information
selenoprotein P
UniProt Protein Name
Selenoprotein P
Protein Family
UniProt Gene Name
SEPP1  [Similar Products]
UniProt Synonym Gene Names
SELP; SeP  [Similar Products]
UniProt Entry Name
SEPP1_HUMAN
NCBI Summary for SEPP1
This gene encodes a selenoprotein containing multiple selenocysteine (Sec) residues, which are encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is an extracellular glycoprotein, and is unusual in that it contains 10 Sec residues per polypeptide. It is a heparin-binding protein that appears to be associated with endothelial cells, and has been implicated to function as an antioxidant in the extracellular space. Several transcript variants, encoding either the same or different isoform, have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for SEPP1
SEPP1: Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis. Belongs to the selenoprotein P family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: extracellular space; extracellular region

Molecular Function: selenium binding

Biological Process: sexual reproduction; locomotory behavior; brain development; response to oxidative stress; selenium metabolic process; growth; post-embryonic development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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