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SERPINE1 elisa kit :: Human PAI-1 ELISA Kit

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Catalog # MBS175945
Unit / Price
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  96-Strip-Wells  /  $455 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

PAI-1 (SERPINE1), ELISA Kit

Popular Item
Full Product Name

Human PAI-1 PicoKine ELISA Kit

Product Synonym Names
Plasminogen activator inhibitor 1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; Endothelial plasminogen activator inhibitor; PAI; PAI-1; PAI1; PAI1_HUMAN; PLANH1; Plasminogen activator inhibitor 1; Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1; Serpin E1; Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1; SERPINE1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
gene 613329
3D Structure
ModBase 3D Structure for P05121
Species Reactivity
Specificity
Natural and recombinant human PAI-1
Samples
Cell culture supernates, Cell lysates, serum and plasma (heparin, EDTA, citrate)
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of SERPINE1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SERPINE1purchase
MBS175945 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PAI-1 (SERPINE1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SERPINE1. The ELISA analytical biochemical technique of the MBS175945 kit is based on SERPINE1 antibody-SERPINE1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SERPINE1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SERPINE1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
SERPINE1 elisa kit
Principle of the assay: human PAI-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PAI-1 has been precoated onto 96-well plates. Standards(sf21, S22-P402) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PAI-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PAI-1 amount of sample captured in plate.
Background: Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots). It is a serine protease inhibitor (serpin) protein (SERPINE1). PAI-1 is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome (7q21.3-q22).1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis (a tendency tohemorrhage). PAI-1 is present in increased levels in various disease states (such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used inthis kit is recombinant human PAI-1 with the molecular mass of 43KDa.

Typical Testing Data/Standard Curve (for reference only) of SERPINE1 elisa kit
Human PAI-1 PicoKine ELISA Kit standard curve
SERPINE1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS175945. Click to request current manual
NCBI/Uniprot data below describe general gene information for SERPINE1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
43,404 Da
NCBI Official Full Name
Plasminogen activator inhibitor 1
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
NCBI Official Symbol
SERPINE1  [Similar Products]
NCBI Official Synonym Symbols
PAI; PAI1; PAI-1; PLANH1
  [Similar Products]
NCBI Protein Information
plasminogen activator inhibitor 1; serpin E1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
UniProt Protein Name
Plasminogen activator inhibitor 1
UniProt Synonym Protein Names
Endothelial plasminogen activator inhibitor; Serpin E1
UniProt Gene Name
SERPINE1  [Similar Products]
UniProt Synonym Gene Names
PAI1; PLANH1; PAI; PAI-1  [Similar Products]
UniProt Entry Name
PAI1_HUMAN
NCBI Summary for SERPINE1
This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
UniProt Comments for SERPINE1
SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: extracellular matrix; extracellular space; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protease binding; receptor binding

Biological Process: circadian rhythm; extracellular matrix organization and biogenesis; positive regulation of blood coagulation; negative regulation of smooth muscle cell migration; defense response to Gram-negative bacterium; positive regulation of receptor-mediated endocytosis; positive regulation of interleukin-8 production; negative regulation of fibrinolysis; platelet degranulation; transforming growth factor beta receptor signaling pathway; regulation of receptor activity; angiogenesis; chronological cell aging; negative regulation of cell migration; negative regulation of cell adhesion mediated by integrin; transcription initiation from RNA polymerase II promoter; platelet activation; transcription, DNA-dependent; negative regulation of blood coagulation; regulation of cell proliferation; fibrinolysis; positive regulation of angiogenesis; gene expression; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; positive regulation of inflammatory response

Disease: Plasminogen Activator Inhibitor-1 Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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